Sequences from confirmation amplicon


Confirmation sequences for line 209F07 and hit At3g59050 are shown.
Line ID 209F07
Locus At3g59050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21825330
Primers used for PCR BE51 - 8474, show primer details
   
Sequence Name 79-K028752-022-209-F07C-8409
GenBank Accession LN513760 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K028752-022-209-F07C-8409
CAGCATCCGCAACAAACGTGGTCTCCTTTTCTCTGTAGTCACCTTCACACCACTGAAGCG
CCTTGAAATCTTGGTAATTCTGACAAAACAAAAGTCCAATA
   
Sequence Name 79-K028752-022-209-F07C-BE51
GenBank Accession LN513761 [GenBank]
Sequencing primer gene specific primer, BE51: 5'-TGCAGCTAAAACCTTATTGGACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K028752-022-209-F07C-BE51
GGCGCTTAGTGGTGTGAAGGTGACTACAGAGAAAGGAGACACGTTTGTTGCGGAAGCTGC
AGTCATTGCTCTTCCTCTAGGAGTCTTAAAGTCAGGAATGATAACATTCGAACCAAAGCT
TCCACAGTGGAAACAAGAAGCTATCAACGATCTCGGTGTTGGAATCGAGAACAAGATCAT
ATTGAATTTTGACAACGTCTTTTGGCCAAACGTAGAGTTTTTAGGAGTCGTTGCAGAAAC
TTCCTACGGATGCAGTTATTTCTTGAACCTGCATAAAGCCACAAGCCATCCTGTCCTTGT
TTACATGCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACAT
Primers used for PCR BE50 - 8474, show primer details
   
Sequence Name 88-K028753-022-209-F07C-8409
GenBank Accession LN514813 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K028753-022-209-F07C-8409
GAAGCCTCTTTACCGGTACAGCTCCAAACTACCTTTTCTTTCACCTGAGTTGCTATATCG
TTTTGCCAATGTACAAGCTCTAATGGGGTTTTCTGTTGGCAGATAAATTATTTGGTATCA
AGGTGGGGATCAGACATCAATTCATTGGGATCATATAGCTATGACATTGTGAACAAACCT
CACGACCTCTATGAGAGACTGAGAGTGCCATTGGATAATCTATTCTTTGCGGGGGAAGCC
ACTAGCTCAAGCTACCCGGGATCAGTGCACGGTGCTTATTCAACCGGAGTGCTGGCAGCT
GAAGACTGCAGGATGCGTGTGCTTGAGCGCTATGGAGAGCTCGAACATGAGATGGAAGAG
GAAGCTCCTGCTTCTGTCCCTCTTCTGATCTCCCGTATGTAATTTAAACCTCAGGTGTTT
TATATCACCTCCATAT
   
Sequence Name 88-K028753-022-209-F07C-BE50
GenBank Accession LN514814 [GenBank]
Sequencing primer gene specific primer, BE50: 5'-CTAACAATGTTCCCATATGGAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K028753-022-209-F07C-BE50
AGCAGGAGCTTCCTCTTCCATCTCATGTTCGAGCTCTCCATAGCGCTCAAGCACACGCAT
CCTGCAGTCTTCAGCTGCCAGCACTCCGGTTGAATAAGCACCGTGCACTGATCCCGGGTA
GCTTGAGCTAGTGGCTTCCCCCGCAAAGAATAGATTATCCAATGGCACTCTCAGTCTCTC
ATAGAGGTCGTGAGGTTTGTTCACAATGTCATAGCTATATGATCCCAATGAATTGATGTC
TGATCCCCACCTTGATACCAAATAATTTATCTGCCAACAGAAAACCCCATTAGAGCTTGT
ACATTGGCAAAACAATATAGCAACTCAGGTGAAAGAAAAGGTAGTTTGGAGCAGTACCGG
TGAAGAGGCATCTGGAAGAATTTTCTGTAGTTGCGAAAAGGCAAAATTTGCAGCTGCTTC
ATCGGACTTTTTCTCGATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11