Sequences from confirmation amplicon


Confirmation sequences for line 211B01 and hit At4g38900 are shown.
Line ID 211B01
Locus At4g38900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18141178
Primers used for PCR DB01 - 8474, show primer details
   
Sequence Name 09-K024722-022-211-B01C-8409
GenBank Accession LN490012 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024722-022-211-B01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGAGTGTCACGGAGGTAAGGA
GTCAAAAGAGAAGAAAGAGTTGGGCTGTGACATTGACCGTGAATGATTAGCTCCTCCCGG
GTTAAAATTATGCGAACCGGGTTGTCGGGTGGTCGGGATCTGAGAAAACGGAGAAGTCGG
TGGGATTAAGTTGGGGTGCGACGGAGGAACACCGATTCGTTTGCCACTGTCACTGAAAGG
CTTGGAGAATTGAGGAGCTGATGAGCGGATGAAATTAGGGTTTAAATCGAGCTGAGAAAT
GGGATTTTTGGGAATGGAAGAAGAAGTAGTGCCGAAAGATGAATGAAGTCTCTGGATCAT
ATCACTGTTACACTTCTCTGTATCACCCATTTTAGATCGGATAATGCAGTTTCTAATTCT
CACCACTCTCCAATAACTAGAGAAAACCCCTAAAA
   
Sequence Name 10-K024722-022-211-B01C-DB01
GenBank Accession LN490485 [GenBank]
Sequencing primer gene specific primer, DB01: 5'-AAGCTTGATCTTTTAGGGGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024722-022-211-B01C-DB01
TAGTTATTGGAGAGTGGTGAGAATTAGAAACTGCATTATCCGATCTAAAATGGGTGATAC
AGAGAAGTGTAACAGTGATATGATCCAGAGACTTCATTCATCTTTCGGCACTACTTCTTC
TTCCATTCCCAAAAATCCCATTTCTCAGCTCGATTTAAACCCTAATTTCATCCGCTCATC
AGCTCCTCAATTCTCCAAGCCTTTCAGTGACAGTGGCAAACGAATCGGTGTTCCTCCGTC
GCACCCCAACTTAATCCCACCGACTTCTCCGTTTTCTCAG

Last Updated on 06.12.2018 16:11