Sequences from confirmation amplicon


Confirmation sequences for line 211D05 and hit At3g18870 are shown.
Line ID 211D05
Locus At3g18870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6508609
Primers used for PCR AK03 - 8474, show primer details
   
Sequence Name 44-K017190-022-211-D05C-AK03
GenBank Accession LN505023 [GenBank]
Sequencing primer gene specific primer, AK03: 5'-TCTCTGTCACTTGCACAAATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017190-022-211-D05C-AK03
CAAAGTAAAATCGGAGAAGACCAATGTACCAAATAGTTTATTATCATTTTTTTGGTGCTA
AAAATATTTAACTTATTACTACAGGTAGCAGAAGAAGAAGCTGCAGATGTTGATTCCAAG
TTCCGGAGTCTGGTAGCAATTGGCTCACTGGTTAGTTTCCGCAACAACTAGAGTAACGTA
ATCCACTTGTTGTTAATTACATAATTTAATCGGTGTGTGTTTGTAACAACAGATGCTTGA
AGGTCTCGTGAAGAAGATAGCGATAGATTTTGATGTAGAGAGCATCGCAAAATCAGCGAA
GGCGTCAAAGGAAGCCAAGATTGCAGAAGTTGGGGCCGATATCGATCTTGTAATTCGTCA
GTAACCTTAAAAGAGAGTCTGTGATCGAACCCGGGATTTGAATTTTCAATGATTCCCTCA
AGAATTTTCATATGATTCAAAATCACTTTTCCTTGAAATGCAATTCAACTTTTTTGTTTT
CAGATTTCTGAATACTAACATATGTTAAAACTTAAAAGATTATGGCTTATGGCTTCCATT
TTGAGTAGAATTTTTGATCACCCCACATTAACATTCTACTAAGCGAAACCCTAACGTTCT
TCTTCTTCAAATGCCAATGTCCAATACTGCGGATATTTTTAAATCTACATCAAATGCGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
   
Sequence Name 48-K017190-022-211-D05C-8409
GenBank Accession LN506362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K017190-022-211-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGCATTTGATGTAGATTT
AAAAATATCCGCAGTATTGGACATTGGCATTTGAAGAAGAAGAACGTTAGGGTTTCGCTT
AGTAGAATGTTAATGTGGGGTGATCAAAAATTCTACTCAAAATGGAAGCCATAAGCCATA
ATCTTTTAAGTTTTAACATATGTTAGTATTCAGAAATCTGAAAACAAAAAAGTTGAATTG
CATTTCAAGGAAAAGTGATTTTGAATCATATGAAAATTCTTGAGGGAATCATTGAAAATT
CAAATCCCGGGTTCGATCACAGACTCTCTTTTAAGGTTACTGACGAATTACAAGATCGAT
ATCGGCCCCAACTTCTGCAATCTTGGCTTCCTTTGACGCCTTCGCTGATTTTGCGATGCT
CTCTACATCAAAATCTATCGCTATCTTCTTCACGAGACCTTCAAGCATCTGTTGTTACAA
ACACACACCGATTAAATTATGTAATTAACAACAAGTGGATTACGTTACTCTAGTTGTTGC
GGAAACTAACCAGTGAGCCAATTGCTACCAGACTCCGGAACTTGGAATCAACATCTGCAG
CTTCTTCTTCTGCTACCTGTAGTAATAAGTTAAATATTTTTAGCACCAAAAAAATGATAA
TAAACTATTTGGTACATTGGTCTTCTCCGATTTTACTTTGATCT

Last Updated on 06.12.2018 16:11