Sequences from confirmation amplicon


Confirmation sequences for line 220E03 and hit At1g27780 are shown.
Line ID 220E03
Locus At1g27780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9678447
Primers used for PCR GD24 - 8474, show primer details
   
Sequence Name 46-K100820-0022-220-E03C-8409
GenBank Accession LN505795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K100820-0022-220-E03C-8409
CATTTACAAACATTATCAGCTGGTTTCACAATATAGTTGACCTCACCCTACACAAATAAG
TATGTTAACCATAGATAATGTACATTTACTTATTATGTACGTACATACACAATTTAACAC
ACCTCATCAGCAGCCTCACACTCCAAAATGCGAGATGTCCGGATTGCTCGCAGAGAGGCA
AGACGATGGATAGACCTCTCAGCAAATGTCCTGTTAGGTACATCATCAGGTCCTAGCTTA
GCAATTGATGGGATTGTTTCGAAAGCTAAAAGCTGGAGAGCTAATGGGAAACCATGGAGT
GCATAAGACCCTACAAGTATACCCCTTATTAGTTTCTGAGCATCGTAAGGAGTTTGGAAA
TTTGCTATCCTCCCCAAAGTACGGGTAAAGGACACCCTTCCCCATGGGTATTTGCAGAAA
AACTCCAGGTTCTTAGTCATCTCGACAGTCTTTGAAGTAGGCCTGTTGGGGTTACTGTGG
GCCGCTACCACACCGTCAACCAAAATAATAAGAGATAGTGCCAGTTGGTTCCACCCCTCC
ATGCTTTCCTCCTCCTGCAGCCAGCTCACCAAATCAGCAATTGTTGGAACCGTGTCCCCA
AACCTCTCATGAAACAGAGTATTCCAAACACTCTCACACTCAGGTT
   
Sequence Name 46-K100832-0022-220-E03C-GD24
GenBank Accession LN505797 [GenBank]
Sequencing primer gene specific primer, GD24: 5'-ATCAGTGTGAAACCTGAGTGTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K100832-0022-220-E03C-GD24
GGGGACACGGTTCCACAATTGCTGATTTGGTGAGCTGGCTGCAGGAGGAGGAAAGCATGG
AGGGGTGGAACCAACTGGCACTATCTCTTATTATTTTGGTTGACGGTGTGGTAGCGGCCC
ACAGTAACCCCAACAGGCCTACTTCAAAGACTGTCGAGATGACTAAGAACCTGGAGTTTT
TCTGCAAATACCCATGGGGAAGGGTGTCCTTTACCCGTACTTTGGGGAGGATAGCAAATT
TCCAAACTCCTTACGATGCTCAGAAACTAATAAGGGGTATACTTGTAGGGTCTTATGCAC
TCCATGGTTTCCCATTAGCTCTCCAGCTTTTAGCTTTCGAAACAATCCCATCAATTGCTA
AGCTAGGACCTGATGATGTACCTAACAGGACATTTGCTGAGAGGTCTATCCATCGTCTTG
CCTCTCTGCGAGCAATCCGGACATCTCGCATTTTGGAGTGTGAGGCTGCTGATGAGGTGT
GTTAAATTGTGTATGTACGTACATAATAAGTAAATGTACATTATCTATGGTTAACATACT
TATTTGTGTAGGTTGAGGTCAACTATATNGTGAAACCAGCTGATAATGTTTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11