Sequences from confirmation amplicon


Confirmation sequences for line 222B10 and hit At3g12110 are shown.
Line ID 222B10
Locus At3g12110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3857843
Primers used for PCR KA14 - 8474, show primer details
   
Sequence Name 49-K105307-0022-222-B10C-KA14
GenBank Accession LN506838 [GenBank]
Sequencing primer gene specific primer, KA14: 5'-ATCATCCCAGTTGCTAACAATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K105307-0022-222-B10C-KA14
TCAAAACCTCTCTTAGACTGAGCCTCATCACCAACGTAAGCATCTTTTTGTCCCATACCA
ACCATGACACCAGTGTGTCGAGGACGACCAACGATGCTAGGGAATACAGCTCTTGGTGCA
TCATCTCCAGCAAATCCAGCCTAAGGAATTGGAAACACATATGAATGAACCAGACAAAAC
AGCATTGTATCCAAAAATAATAAAAGAAAAAGTTATCAGATTTATATCCACAAAGGTCAT
GTAAAGATGTACCTTAACCATTCCGGTTCCATTGTCACAGACGAGAGGCTGAATGTCTTC
ACCATCTGCCATTTTCTATATCCTGTCAAAATTGATATAAAAAATATTAGCATAATTGAA
CCCTTTATTTTGCAAGAAACAGAGGAATCTTAAGATCTACCAAAGAGAATTAAATTGAAA
AAGCTTCATAAACGCATACTAATGAAGAATCAATGACGATGGAACGTTATGGAAATGTGA
TTACCTTAGAAGTTAGAGAGGGGATCTCTGAGGAAACGAGGAGGGTGGTGGTGTGGTACG
GGTGTGTAGTTTTTGAGAGAGAAACGGAGCGAATGAAGAAGAGAAAAAGAAATGAAATGA
AATGAAATGAAATATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 33-K105308-0022-222-B10C-8409
GenBank Accession LN500992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K105308-0022-222-B10C-8409
ATTTACAATTGAATATATCCATATTTCATTTCATTTCATTTCATTTCTTTTTCTCTTCTT
CATTCGCTCCGTTTCTCTCTCAAAAACTACACACCCGTACCACACCACCACCCTCCTCGT
TTCCTCAGAGATCCCCTCTCTAACTTCTAAGGTAATCACATTTCCATAACGTTCCATCGT
CATTGATTCTTCATTAGTATGCGTTTATGAAGCTTTTTCAATTTAATTCTCTTTGGTAGA
TCTTAAGATTCCTCTGTTTCTTGCAAAATAAAGGGTTCAATTATGCTAATATTTTTTATA
TCAATTTTGACAGGATATAGAAAATGGCAGATGGTGAAGACATTCAGCCTCTCGTCTGTG
ACAATGGAACCGGAATGGTTAAGGTACATCTTTACATGACCTTTGTGGATATAAATCTGA
TAACTTTTTCTTTTATTATTTTTGGATACAATGCTGTTTTGTCTGGTTCATTCATATGTG
TTTCCAATTCCTTAGGCTGGATTTGCTGGAGATGATGCACCAAGAGCTGTATTCCCTAGC
ATCGTTGGTCGTCCTCGACACACTGGTGTCATGGTTGGTATGGGACAAAAAGATGCTTAC
GTTGGTGATGAGGCTCAGTCTAAGAGAGGTATTTTGACATTGAAATATCCTATTGAGCAT
GGTATTGTTAGCACGGGGGAATG

Last Updated on 06.12.2018 16:11