Sequences from confirmation amplicon


Confirmation sequences for line 224D07 and hit At4g16210 are shown.
Line ID 224D07
Locus At4g16210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9178052
Primers used for PCR YP06 - 8474, show primer details
   
Sequence Name 35-K019979-022-224-D07C-YP06
GenBank Accession LN501646 [GenBank]
Sequencing primer gene specific primer, YP06: 5'-TCAAACACAAAACAGAGGATTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019979-022-224-D07C-YP06
TATCATCAAAAACATTCATCTCAAGGCAACAAAATTTTGAGAGCATAAACAAATGAGATT
GAATTTTAAGATGTTGAATTGAGTAGAGCTTGAAACAACATGATTTTCTATGATCCAAAG
CTAAAATCTGCAACTATATTTGACCTAATTGCTAGATACCAGTGCTATTGATAACTATGC
AAACGACGTCGTAAGAGAAGAAATCAACCTGGCGTGAGTATCCATGAACTTAGCTCCTCT
AGAAGCGACCAAAATATCACAGGCCAAGGCGAGTTCAAACCCGGCGGTGATGGCAAAACC
GTTAATAGCTCCGATGATCGGTTTACGTAACCGCTCCATCTGCACAACCGGGTCGGTTTC
CGGATCCTTCACGTCTCCTTTGAAAACAGACTCCGCCGCAGTCAAATCAACGCCAGAGCA
GAAAGATCGACCTGATCCGGTGAAAATCACGACCTGGACCGATTCGTCGGAGTCCATATC
CTTGAATGCCTTGGCGAGATCAACCATCATCGCTCTCGTGAGTGAATTGAGAGATTTCGG
ACGGTTGATTGTGATGACGGCGATCCCGCCTGATTCTTTCTTCACCTGAATGAGATTTTC
CGATACTGTTTGATCCATTTTTCGACGATTCCGATTACAATTGAACGGATTTCGAATTTT
CCGATCGGGTTTTTAAATAGAGTCAATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCACCAATGAGTATG
   
Sequence Name 39-K019979-022-224-D07C-8409
GenBank Accession LN503197 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K019979-022-224-D07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATTGACTCTATTTAAA
AACCCGATCGGAAAATTCGAAATCCGTTCAATTGTAATCGGAATCGTCGAAAAATGGATC
AAACAGTATCGGAAAATCTCATTCAGGTGAAGAAAGAATCAGGCGGGATCGCCGTCATCA
CAATCAACCGTCCGAAATCTCTCAATTCACTCACGAGAGCGATGATGGTTGATCTCGCCA
AGGCATTCAAGGATATGGACTCCGACGAATCGGTCCAGGTCGTGATTTTCACCGGATCAG
GTCGATCTTTCTGCTCTGGCGTTGATTTGACTGCGGCGGAGTCTGTTTTCAAAGGAGACG
TGAAGGATCCGGAAACCGACCCGGTTGTGCAGATGGAGCGGTTACGTAAACCGATCATCG
GAGCTATTAACGGTTTTGCCATCACCGCCGGGTTTGAACTCGCCTTGGCCTGTGATATTT
TGGTCGCTTCTAGAGGAGCTAAGTTCATGGATACTCACGCCAGGTTGATTTCTTCTCTTA
CGACGTCGTTTGCATAGTTATCAATAGCACTGGTATCTAGCAATTAGGTCAAATATAGTT
GCAGATTTTAGCTTTGGATCATAGAAAATCATGTTGTTTCAAGCTCTACTCAATTCAACA
TCTTAAAATTCAATCTCATTTGTTTATGCTCTCAAAATTTTGTTGCCTTGAGATGAATGT
TTTTGATGATATGAATGTG

Last Updated on 06.12.2018 16:11