Sequences from confirmation amplicon


Confirmation sequences for line 227G05 and hit At5g43290 are shown.
Line ID 227G05
Locus At5g43290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17372698
Primers used for PCR IS26 - 8474, show primer details
   
Sequence Name 27-K017400-022-227-G05C-IS26
GenBank Accession LN498165 [GenBank]
Sequencing primer gene specific primer, IS26: 5'-CCTAATTGTGCTTCTTTGCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K017400-022-227-G05C-IS26
TATGTTTATGTATTTTCGTTTTTTTATTGGGCCATTGACGTGTCTTATCGGGTAGGAAGA
AAGGGTAAGTATAGTGGAAGTGGAAACCTTCGTAGGTAATGATATATGTGTTAGACTCAT
CAATTGATCTCTCCACTTGCTTCTTTGCATTGCATATTGGGTTTGTGCACTTGTAATAAC
TCCTAGACATATATCACAAATATAGTAACATTAGGCTTGTGACCGAAAACTAAGTATACT
AAATAAAACTTCCATATGTTGATTTTAATACGTATCATTTTGGCCGAACCAAAGAAGTTC
GATATGCATATACAATTCTAAAACGGAGAATGCATATATGTACCTTGGGTTAGGGCTATT
TTTGATTGATTTTTGGCCATATTTTCTCCATTTGTATCCATCATCACACATTCCATTACT
ATTGTTCTTCACCTTTAAAGTGTACCTATCCACCTTGCTCAAAGTACTTCAGTATTGAAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCT
   
Sequence Name 28-K017400-022-227-G05C-8409
GenBank Accession LN498634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K017400-022-227-G05C-8409
AGATTTTCCGGACATGAAGCCATTTACAATTGAATATATTCAATACTGAAGTACTTTGAG
CAAGGTGGATAGGTACACTTTAAAGGTGAAGAACAATAGTAATGGAATGTGTGATGATGG
ATACAAATGGAGAAAATATGGCCAAAAATCAATCAAAAATAGCCCTAACCCAAGGTACAT
ATATGCATTCTCCGTTTTAGAATTGTATATGCATATCGAACTTCTTTGGTTCGGCCAAAA
TGATACGTATTAAAATCAACATATGGAAGTTTTATTTAGTATACTTAGTTTTCGGTCACA
AGCCTAATGTTACTATATTTGTGATATATGTCTAGGAGTTATTACAAGTGCACAAACCCA
ATATGCAATGCAAAGAAGCAAGTGGAGAGATCAATTGATGAGTCTAACACATATATCATT
ACCTACGAAGGTTTCCACTTCCACTATACTTACCCTTTCTTCCTACCCGATAAGACACGT
CAATGGCCCAATAAAAAAACGAAAATACATAAACATAATGCTCAAGATATGAACAAAAAA
TCTCAAACCCAAGAAGAGAGCAAAGAAGCCCCAT

Last Updated on 06.12.2018 16:11