Sequences from confirmation amplicon


Confirmation sequences for line 227H06 and hit At3g17220 are shown.
Line ID 227H06
Locus At3g17220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5883715
Primers used for PCR RD01 - 8474, show primer details
   
Sequence Name 49-K019824-022-227-H06C-RD01
GenBank Accession LN506683 [GenBank]
Sequencing primer gene specific primer, RD01: 5'-ACTAAATGTCATTCACGCTGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K019824-022-227-H06C-RD01
GTAACTTAAAGACCAATAAATGAATATATTCATAGTCAAAATTACCGTGTCTTGCATCTC
AGCTAAGACTTTAATCTCTCGTTACTTCTGGTGTATATTTATGACAATTATCTCCATAGA
ACCTCTTCCTACAGTAATCTTTCTTAGCACCAACAAATCATGAACACATATTAGTGAATG
CATATCTACCTAAAGAAGAGAAGTCAGGAAAGTAAGTTAGACGAATACTAATCAGATAAC
TGTCTCTGCACTAATGCTAAGAATAGCAAAGTTTTCTCTCTCTCTTACATAAGGACTTGT
GGTCTAACTAAGAATCAGAAAGAGTAACAACCCATTTCTATTATTAGTTCTAGTGGAAGC
AATCAATCGCATGCATCTATAAATATGAATAGTCAGGCATAGTAAGGGAATAACTTAACC
ATCATCTACCAACGAACATAAGAAAGAAAGAAGCAAAGATGGCAGCATACCTGACGAACA
GAGTTTTAATGTCTTCTCTGATGTTTTTTGTAATGACTGGTTCTTTGAACGCACAAGTGG
CAGACATAAAAGCGATATGTGGAAAAGCGAAAAACCAATCCTTCTGTACGAGCAAATACA
AATACATACTAAGAAACAATGGATCAGCCGCTCAAAAATGGATCAGCAATGAGTATGATG
GTCAATATNGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
   
Sequence Name 50-K019824-022-227-H06C-8409
GenBank Accession LN507028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K019824-022-227-H06C-8409
ATCCATTTTTGAGCGGCTGATCCATTGTTTCTTAGTATGTATTTGTATTTGCTCGTACAG
AAGGATTGGTTTTTCGCTTTTCCACATATCGCTTTTATGTCTGCCACTTGTGCGTTCAAA
GAACCAGTCATTACAAAAAACATCAGAGAAGACATTAAAACTCTGTTCGTCAGGTATGCT
GCCATCTTTGCTTCTTTCTTTCTTATGTTCGTTGGTAGATGATGGTTAAGTTATTCCCTT
ACTATGCCTGACTATTCATATTTATAGATGCATGCGATTGATTGCTTCCACTAGAACTAA
TAATAGAAATGGGTTGTTACTCTTTCTGATTCTTAGTTAGACCACAAGTCCTTATGTAAG
AGAGAGAGAAAACTTTGCTATTCTTAGCATTAGTGCAGAGACAGTTATCTGATTAGTATT
CGTCTAACTTACTTTCCTGACTTCTCTTCTTTAGGTAGATATGCATTCACTAATATGTGT
TCATGATTTGTTGGTGCTAAGAAAGATTACTGTAGGAAGAGGTTCTATGGAGATAATTGT
CATAAATATACACCAGAAGTAACGAGAGATTAAAGTCTTAGCTGAGATGCAAGACACGGT
AATTTTGACTATGAATATATTCATTTATTGGTCTTTAAGTTACTAGTACATCAG

Last Updated on 06.12.2018 16:11