Sequences from confirmation amplicon


Confirmation sequences for line 228D04 and hit At1g29280 are shown.
Line ID 228D04
Locus At1g29280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10237496
Primers used for PCR IS28 - 8474, show primer details
   
Sequence Name 51-K017403-022-228-D04C-IS28
GenBank Accession LN507343 [GenBank]
Sequencing primer gene specific primer, IS28: 5'-TTCTCATTCGCCTAAAAGAAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K017403-022-228-D04C-IS28
AAAAATTCTCATTTGACTAAAGTATATTCATATATTGATGTACTTGTTACTTTACAGCAG
GAGATCCGTAGAGAAGAGAGTAGTGAACGTACCGATGAAAGAAATGGAAGGATCTCGGCA
CAAAGGGGACACAACTCCGCCCTCAGATTCATGGGCTTGGCGTAAGTACGGCCAAAAGCC
CATTAAGGGATCTCCATACCCTAGAGGTTATTACCGTTGTAGTAGCACAAAAGGTTGTCC
GGCGAGGAAGCAAGTCGAGAGAAGCAGAGATGATCCAACGATGATTCTCATCACTTACAC
TTCTGAGCATAACCATCCTTGGCCTCTCACTTCTTCCACAAGAAACGGGCCTAAACCTAA
ACCCGAGCCCAAACCCGAACCTGAGCCTGAGGTAGAGCCAGAGGCGGAGGAGGAGGATAA
TAAGTTTATGGTTCTTGGAAGGGGGATTGAAACGACGCCATCATGTGTCGACGAGTTCGC
ATGGTTTACGGAGATGGAGACAACATCTTCGACGATTCTTGAGAGCCCGATTTTCTCGTC
GGAGAAAAAGACGGCTGTCTCGGGGGCTGATGACGTGGCGGTGTTCTTTCCGATGGGAGA
AGAGGATGAGTCTTTGTTCGCCGATCTCGGCGAGTTGCCGGAGTGTTCTGTGGTGTTTCG
TC
   
Sequence Name 52-K017403-022-228-D04C-8409
GenBank Accession LN507666 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K017403-022-228-D04C-8409
ACATGAAGCCATTTACAATTGAATAATATCCAATAAAATATCACCACGTGTCAAAAGATC
TCAACTTGTGACCCCACTACGCTACTCCGGTGACGAAACACCACAGAACACTCCGGCAAC
TCGCCGAGATCGGCGAACAAAGACTCATCCTCTTCTCCCATCGGAAAGAACACCGCCACG
TCATCAGCCCCCGAGACAGCCGTCTTTTTCTCCGACGAGAAAATCGGGCTCTCAAGAATC
GTCGAAGATGTTGTCTCCATCTCCGTAAACCATGCGAACTCGTCGACACATGATGGCGTC
GTTTCAATCCCCCTTCCAAGAACCATAAACTTATTATCCTCCTCCTCCGCCTCTGGCTCT
ACCTCAGGCTCAGGTTCGGGTTTGGGCTCGGGTTTAGGTTTAGGCCCGTTTCTTGTGGAA
GAAGTGAGAGGCCAAGGATGGTTATGCTCAGAAGTGTAAGTGATGAGAATCATCGTTGGA
TCATCTCTGCTTCTCTCGACTTGCTTCCTCGCCGGACAACCTTTTGTGCTACTACAACGG
TAATAACCTCTAGGGTATGGAGATCCCTTAATGGGCTTTTGGCCGTACTTACGCCAAGCC
CATGAATCTGAGGGCGGAGTTGTGTCCCCTTTGTGCCGAGATCCTTCCATTTCTTTCATC
GGTACGTTCACTACTCTCTTCTCTACGGATCTCCTGCTGTAAAGTAACAAGTACATCAAT
ATATGAATATACTTTAGTCAAATGAGAATTTTTCTTGAAATTGTTATATAATTCGAATCA
CATATATCTACCTACCTT

Last Updated on 06.12.2018 16:11