Sequences from confirmation amplicon


Confirmation sequences for line 230B04 and hit At5g63120 are shown.
Line ID 230B04
Locus At5g63120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25318837
Primers used for PCR FA51 - 8474, show primer details
   
Sequence Name 63-K037324-0022-230-B04C-8409
GenBank Accession LN510928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K037324-0022-230-B04C-8409
GAGCCATTTACATTGAATATTTCCCGGACTGCGCTGCTTCTTCTTCTTCTTCTTCATGAT
ATTTCTAAACATTTTGCCTTTTTCTTAATTCTATCAACTAAATACACTTTACTACTTTAC
TCTCAAGTCAACCGTAACACTGTAATGAAACTTGTTTTTAAAAATCGGATGTGGATTATT
AGGTTGGGAGATTCAAGTATTGCGAATAGAATTTAGACAAATGTAAGCATCTATAACATC
TAGAACATGTATATCTTCTCTTTTTTCCCAACAACATCTAGTTACGAATCCTTAAGGTGA
AATGTGTAAATAGATCATACTTAGCTAAAAAACCCTTTACATTTATTTGTCTAACATTGT
CTACCATTAGGAAAACAAGTTAAAAATAATTCGCTATGTTTGAATCGAATTTGGAATTAT
ATAATCGATCGTTAGTTTAACATTCAGTAATTATTAAAATTAAATTTTTAACGCAAAGAT
AATAATGTAAGTAATCATTAATGTGCGTGTATATATAAGCTGTGCCCACAACTCCTAGTA
ATGACTTTCTCAGCCATTGTT
   
Sequence Name 55-K037324-0022-230-B04C-FA51
GenBank Accession LN508737 [GenBank]
Sequencing primer gene specific primer, FA51: 5'-CAACAATGCCTGAGAAAGTCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K037324-0022-230-B04C-FA51
GCTTATATATACACGCACATTAATGATTACTTACATTATTATCTTTGCGTTAAAAATTTA
ATTTTAATAATTACTGAATGTTAAACTAACGATCGATTATATAATTCCAAATTCGATTCA
AACATAGCGAATTATTTTTAACTTGTTTTCCTAATGGTAGACAATGTTAGACAAATAAAT
GTAAAGGGTTTTTTAGCTAAGTATGATCTATTTACACATTTCACCTTAAGGATTCGTAAC
TAGATGTTGTTGGGAAAAAAGAGAAGATATACATGTTCTAGATGTTATAGATGCTTACAT
TTGTCTAAATTCTATTCGCAATACTTGAATCTCCCAACCTAATAATCCACATCCGATTTT
TAAAAACAAGTTTCATTACAGTGTTACGGTTGACTTGAGAGTAAAGTAGTAAAGTGTATT
TAGTTGATAGAATTAAGAAAAAGGCAAAATGTTTAGAAATATCATGAAGAAGAAGAAGAA
GAAGCAGCGCAGTCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACTTCAC

Last Updated on 06.12.2018 16:11