Sequences from confirmation amplicon


Confirmation sequences for line 233H06 and hit At3g03200 are shown.
Line ID 233H06
Locus At3g03200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:737876
Primers used for PCR MZ09 - 8474, show primer details
   
Sequence Name 53-K019551-022-233-H06C-MZ09
GenBank Accession LN508002 [GenBank]
Sequencing primer gene specific primer, MZ09: 5'-AACGGTCTAGAAATCGAACTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K019551-022-233-H06C-MZ09
TTACCAGGTAATATTAATTTGACGTTTCCCTTGTTTATGCATGAGATCCTCTTAATCAAA
TATGATTTAAATTTTTTGAAAAATATGATTTCTGATCAACATTAATTACGCGATTCTACT
TTAGTTATCAAATTCTGCATATATTATTCATTTATATGGATTTAAATTACAACATCTGTA
ATCGTTAAGAATTATTATTATTTGTCTAAAACCCATAGGAACAAGAGAGATTGAAGAAAA
GAATAAGAATATAGCATTGATGAAGAAACCCTAAATATACTTTAGTCTCTACCGGGAAAA
TACCACATGTGCAATTACACATACTAGTTCTGTAAATAACTATTTTATTATTTTTTTCAT
CATTTTCACCAAATCTTTTAGGAATTAAAATGTCAAAATAATTTTTAAAAATGTTTAAAT
ATAAAATTGTAACTCATGAAAACATGAATGCCAGCTTAATTGAATCATAATTTTAATTTA
CGTCATCCAATATCTGCTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATTCACAATGAGTATGATGGTCCATATGGAGAAAAAGAAAGAGTAATTACCCATTT
TTTTTCAATTCAAAAAATGTA
   
Sequence Name 54-K019551-022-233-H06C-8409
GenBank Accession LN508336 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K019551-022-233-H06C-8409
CTCCGGACATGAAGCCATTTACAATTGAATATATCAGCAGATATTGGATGACGTAAATTA
AAATTATGATTCAATTAAGCTGGCATTCATGTTTTCATGAGTTACAATTTTATATTTAAA
CATTTTTAAAAATTATTTTGACATTTTAATTCCTAAAAGATTTGGTGAAAATGATGAAAA
AAATAATAAAATAGTTATTTACAGAACTAGTATGTGTAATTGCACATGTGGTATTTTCCC
GGTAGAGACTAAAGTATATTTAGGGTTTCTTCATCAATGCTATATTCTTATTCTTTTCTT
CAATCTCTCTTGTTCCTATGGGTTTTAGACAAATAATAATAATTCTTAACGATTACAGAT
GTTGTAATTTAAATCCATATAAATGAATAATATATGCAGAATTTGATAACTAAAGTAGAA
TCGCGTAATTAATGTTGATCAGAAATCATATTTTTCAAAAAATTTAAATCATATTTGATT
AAGTGGATCTCATGCATAAACAAGGGAAACGTCAAATTAATATTACCTGGTAAGTCCCAT
GGCTCACACTTGTAAAGATCAACTTCAGCGATAACTTCAAGTTCGATTT

Last Updated on 06.12.2018 16:11