Sequences from confirmation amplicon


Confirmation sequences for line 240H04 and hit At5g39390 are shown.
Line ID 240H04
Locus At5g39390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15764453
Primers used for PCR PP17 - 8474, show primer details
   
Sequence Name 09-K102864-0022-240-H04C-8409
GenBank Accession LN490129 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K102864-0022-240-H04C-8409
CATTTACATTGAATATATCCTGGAAATGTAGATATGTGGCTACCGCCAGAAGATTTGGAA
AGTGCAAACAATCACTCAAGGTCTTTAACATTTGCAGAGAAAGTCAACATAGCAATAGAT
GTGGCTTCTGCTTTGGAGTATCTGCATGTTTATTGTCATGACCCTGTCGCTCATTGTGAT
ATTAAGCCAAGCAACGTTCTTCTAGACGATGATTTGACGGCTCATGTTAGTGACTTTGGT
TTGGCTCGGCTCCTTTATAATTTCGATGAGAAAACCTTTCTAAACCAGTTCAGTTCTGCC
GGTGTGAGAGGTACCATTGGCTATGCCGCACCAGGTATAAACCAAAACTCTGTATGCTAA
ATAGGATTGTTTATAATTAACTCTGAATGATCCTATCTTAATATCTATTGTTTCTTTGAC
ATATGTGTAGAATATGGAATGGGAAGCAA
   
Sequence Name 09-K102869-0022-240-H04C-PP17
GenBank Accession LN490130 [GenBank]
Sequencing primer gene specific primer, PP17: 5'-TTTGCTTCCCATTCCATATTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K102869-0022-240-H04C-PP17
ATACCTGGTGCGGCATAGCCAATGGTACCTCTCACACCGGCAGAACTGAACTGGTTTAGA
AAGGTTTTCTCATCGAAATTATAAAGGAGCCGAGCCAAACCAAAGTCACTAACATGAGCC
GTCAAATCATCGTCTAGAAGAACGTTGCTTGGCTTAATATCACAATGAGCGACAGGGTCA
TGACAATAAACATGCAGATACTCCAAAGCAGAAGCCACATCTATTGCTATGTTGACTTTC
TCTGCAAATGTTAAAGACCTTGAGTGATTGTTTGCACTTTCCAAATCTTCTGGCTGTAGC
CACATATCTACATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
Primers used for PCR KH46 - 8474, show primer details
   
Sequence Name 40-K118534-0022-240-H04C-KH46
GenBank Accession LN503873 [GenBank]
Sequencing primer gene specific primer, KH46: 5'-TCTTGGTTCAAGAAGAAGAACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118534-0022-240-H04C-KH46
GGGATGTTTTTTGAGAAGATAGTTATGAAGAGCTTTATAATGCAACAAGTGGCTTCTCTT
CAAGCAATCTAATTGGTTCAGGCAACTTCAGTGATGTGTTTAAAGGATTGCTTGGCCTCG
AGGAAAAACTCGTCGCGGTTAAAGTTTTGAACCTCCTGAAACATGGAGCAACAAAAAGCT
TTATAGCGGAATGTGAATCTTTCAAAGGTATTAGGCACCGTAACCTTGCGAAACTGATAA
CAGTTTGCTCAAGCCTTGATTCCCAAGGAAATGATTTCAGAGCTTTAGTCTATGAGCAAT
GAGTGAATATATGATCATGTACAAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGGTTATTAT
   
Sequence Name 19-K118533-0022-240-H04C-8409
GenBank Accession LN494969 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118533-0022-240-H04C-8409
CATTTCAATTGGTAATTTGTACATGATCATATATTCACTCATTGCTCATAGACTAAAGCT
CTGAAATCATTTCCTTGGGAATCAAGGCTTGAGCAAACTGTTATCAGTTTCGCAAGGTTA
CGGTGCCTAATACCTTTGAAAGATTCACATTCCGCTATAAAGCTTTTTGTTGCTCCATGT
TTCAGGAGGTTCAAAACTTTAACCGCGACGAGTTTTTCCTCGAGGCCAAGCAATCCTTTA
AACACATCACTGAAGTTGCCTGAACCAATTAGATTGCTTGAAGAGAAGCCACTTGTTGCA
TTATAAAGCTCTTCATAACTTATCTTCTCAAAAAACATCCCCACAGTAGTAGAATCAGAT
GGATTAGTATACTACCACTAACATCGTTCTTCTTCTGAAACCAAGA

Last Updated on 06.12.2018 16:11