Sequences from confirmation amplicon


Confirmation sequences for line 243B11 and hit At5g45300 are shown.
Line ID 243B11
Locus At5g45300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18356268
Primers used for PCR AK09 - 8474, show primer details
   
Sequence Name 17-K017369-022-243-B11C-AK09
GenBank Accession LN493656 [GenBank]
Sequencing primer gene specific primer, AK09: 5'-TGTTCTTCCAAGAAAGAGGTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K017369-022-243-B11C-rk09
TTAAATTGCTACTCACAATTGCTAATTGGTCATGCTGAGAATGTTCTGTCTCTTGCAAAT
CTCGCATTTGAGGAGACGAAGATTATTGTCAAGGTATAATCCTTTTGGCACTTATCCTTC
AAACCCAGTGATGCCTATAACAATGAGACTCATACACCAAAAACAACAATGCAGATACCG
GCAATATACTGGTCATACAAGACAGCTAGTCATGCTGCTGAATTAACTGCAGGATACTAC
AACCCTTCAAACCGTGATGGGTACTCTCTTGTTTTCGAAACTCTGAAGAAATATTCCGTG
ACTGTGAAGTTTGTGTGCCCTGGGCCACAAATGTCTCCCAATGCGCATGAAGAAGCATTA
GCTGATCCAGAAGGTTTAAGTTAGCTGATCCAGAAAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 21-K017369-022-243-B11C-8409
GenBank Accession LN495455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017369-022-243-B11C-8409
AAAGCCATTTCCAATTGAATATATCCTTTCTGGATCAGCTAACTTAAACCTTCTGGATCA
GCTAATGCTTCTTCATGCGCATTGGGAGACATTTGTGGCCCAGGGCACACAAACTTCACA
GTCACGGAATATTTCTTCAGAGTTTCGAAAACAAGAGAGTACCCATCACGGTTTGAAGGG
TTGTAGTATCCTGCAGTTAATTCAGCAGCATGACTAGCTGTCTTGTATGACCAGTATATT
GCCGGTATCTGCATTGTTGTTTTTGGTGTATGAGTCTCATTGTTATAGGCATCACTGGGT
TTGAAGGATAAGTGCCAAAAGGATTATACCTTGACAATAATCTTCGTCTCCTCAAATGCG
AGATTTGCAAGAGACAGAACATTCTCAGCATGACCAATTAGCAATTGTGAGTACCAATTT
AAGAAGAAGCGGCCATAATAGCTGTCATATTCACCTCTTTCTTGGAAGAACA

Last Updated on 06.12.2018 16:11