Sequences from confirmation amplicon


Confirmation sequences for line 244D12 and hit At5g14870 are shown.
Line ID 244D12
Locus At5g14870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4809744
Primers used for PCR UK15 - 8474, show primer details
   
Sequence Name 17-K019977-022-244-D12C-UK15
GenBank Accession LN493672 [GenBank]
Sequencing primer gene specific primer, UK15: 5'-ATCAATGTTCAAAAATCGTTAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K019977-022-244-D12C-UK15
GAAGATTACAGAACAGAGATATTTTGGGAGCCTCATGCTTTGTTTCTGACTATCTTTTGA
TCTCAGCTTGTTATTTGGCTAGTAATTCCAGCGGCCACAAATGGAACTGCTAATCATGCC
AATAGCACTCTCGCACTTATCGTTCTTGTTCAATACATTCCAAGATCATTCATCATATTC
CCACTCAACCAAAGGATTATCAAAACAACCGGGTTTATCGCCAAGACTGCTTGGGCAGGA
GCTGCATACAACCTCCTATTGTACATCCTTGCTAGTCACGTAAGTTTTCTTTATCTTGTG
TGTTTTTTCAAATCTTTAGGTTTCTTCAGTTTTAAGTTGCTAAGAATCTCTATTTGTGGG
CAGGTGTTAGGAGCAATGTGGTATCTGTCATCAATCGGGCGGCAGTTTTCGTGCTGGTCC
AACGTATGCAAGAAAGACAACGCTTTGAGAGTTTTGGATTGTCTTCCTAGCTTCTTGGAT
TGCAAGAGTTTAGAACAACCAGAGCGCCAGTATTGGCAGAACGTTACTCAGGTCCTTTCT
CATTGTGATGCTACAAGCAGCACTACCAATTTCAAATTCGGAATGTTTGCTGAATAGAGA
GACCTAACATGGCTCTAGAGTGAATGTTTTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTG
   
Sequence Name 21-K019977-022-244-D12C-8409
GenBank Accession LN495471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K019977-022-244-D12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAACATTCACTCTAGA
GCCATGTTAGGTCTCTCTATTCAGCAAACATTCCGAATTTGAAATTGGTAGTGCTGCTTG
TAGCATCACAATGAGAAAGGACCTGAGTAACGTTCTGCCAATACTGGCGCTCTGGTTGTT
CTAAACTCTTGCAATCCAAGAAGCTAGGAAGACAATCCAAAACTCTCAAAGCGTTGTCTT
TCTTGCATACGTTGGACCAGCACGAAAACTGCCGCCCGATTGATGACAGATACCACATTG
CTCCTAACACCTGCCCACAAATAGAGATTCTTAGCAACTTAAAACTGAAGAAACCTAAAG
ATTTGAAAAAACACACAAGATAAAGAAAACTTACGTGACTAGCAAGGATGTACAATAGGA
GGTTGTATGCAGCTCCTGCCCAAGCAGTCTTGGCGATAAACCCGGTTGTTTTGATAATCC
TTTGGTTGAGTGGGAATATGATGAATGATCTTGGAATGTATTGAACAAGAACGATAAGTG
CGAGAGTGCTATTGGCATGATTAGCAGTTCCATTTGTGGCCGCTGGAATTACTAGCCAAA
TAACAAGCTGAGATCAAAAGATAGTCAGAAACAAAGCATGAGGCTCCCAAAATATCTCTG
TTCTTTAATCTTCTAGCTACCGCCTAACGATT

Last Updated on 06.12.2018 16:11