Sequences from confirmation amplicon


Confirmation sequences for line 244E08 and hit At4g23390 are shown.
Line ID 244E08
Locus At4g23390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12219308
Primers used for PCR GD32 - 8474, show primer details
   
Sequence Name 06-K100850-0022-244-E08C-8409
GenBank Accession LN488727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100850-0022-244-E08C-8409
CTTATAGAATGGTTCTTGAGCAAAGGATGATCAAAAGCTAGCTGTTTTTGAATGTCGATG
CAGTCAAATATATAACCATGTTCAGTCTGTCGAAATCAAAAGCAAGAAAAAATTGGTGTG
GTCTGAAAATAAATGTAACTTGATGATAGTTGTAATATTGTTACAATAGTCAACAATTTT
TGTAATGTTTTATGAAAATTTAAGAAAAGAAAGGCTAAATTTACTTGGAAACTTTTGACT
GCAGGTTTATTGAGATAGGTAAGTAGCTTCTTCATCTCTTCTTTCTCCTCTTTCGA
   
Sequence Name 06-K100856-0022-244-E08C-GD32
GenBank Accession LN488728 [GenBank]
Sequencing primer gene specific primer, GD32: 5'-TCGAAAGAGGAGAAAGAAGAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100856-0022-244-E08C-GD32
TGCAGTCAAAGTTTCCAGTAAATTTAGCCTTTCTTTTCTTAAATTTTCATAAAACATTAC
AAAAATTGTTGACTATTGTAACAATATTACAACTATCATCAAGTTACATTTATTTTCAGA
CCACACCAATTTTTTCTTGCTTTTGATTTCGACAGACTGAACATGGTTATATATTTGACT
GCATCGACATTCAAAAACAGCTAGCTTTTGATCATCCTTTGCTCAAGAACCATTCTATTA
AGGTTTATCCTACAAACCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11