Sequences from confirmation amplicon


Confirmation sequences for line 244E11 and hit At5g06600 are shown.
Line ID 244E11
Locus At5g06600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2023478
Primers used for PCR HV36 - 8474, show primer details
   
Sequence Name 79-K033098-022-244-E11C-8409
GenBank Accession LN513768 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K033098-022-244-E11C-8409
AACTACAGACTTTTTTAGTCAGCACAATTTTGAAGGTTTCTCATGAAATCCAGATCAATT
GTTACCAGAAAGACCGTTTTCACATACACTAATTTATTTCCTTTTTGGAAAAAATAAATC
AAAGATACGCAGGCAAGTACCTTAAACTGTTGAAATGGTGTCTGTTTCTGGATACGGAAA
CTGCGAACTTTGTCATGATCCACAAGATCAAAATATATATCCTTCCCAATTTGTTCCTTA
AGGTCTTCATCTCTTGCAACCTTTCATGTTAACAAAGATTATGTTAGTAACGACAGTATC
AAAATAACACTCTGGAACAGTTCAAAAGATATTATCAGGCCCAATTAAATAGGATAAACA
AACCTTAATTATGGTATATAAGTGAGCTTGTGCCTTGTATCTTCTTTTATCTTCCTTTTC
TTCTTGCTCTTTCTTTAGCCTCACTGGTCAAAA
   
Sequence Name 31-K033098-022-244-E11C-HV36
GenBank Accession LN500055 [GenBank]
Sequencing primer gene specific primer, HV36: 5'-ATTTTGACAGGTGAGGCTAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K033098-022-244-E11C-HV36
GCACAAGCTCACTTATATACCATAATTAAGGTTTGTTTATCCTATTTAATTGGGCCTGAT
AATATCTTTTGAACTGTTCCAGAGTGTTATTTTGATACTGTCGTTACTAACATAATCTTT
GTTAACATGAAAGGTTGCAAGAGATGAAGACCTTAAGGAACAAATTGGGAAGGATATATA
TTTTGATCTTGTGGATCATGACAAAGTTCGCAGTTTCCGTATCCAGAAACAGACACCATT
TCAACAGTTTAAGGTACTTGCCTGCGTATCTTTGATTTATTTTTTCCAAAAAGGAAATAA
ATTAGTGTATGTGAAAACGGTCTTTCTGGTAACAATTGATCTGGATTTCATGAGAAACCT
TCAAAATTCTGCTGACTAAAAAAGTTCTTTAGTTTAATATCTATTGATCCTTGTTACAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATNTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11