Sequences from confirmation amplicon


Confirmation sequences for line 245F03 and hit At3g19760 are shown.
Line ID 245F03
Locus At3g19760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6866205
Primers used for PCR BA30 - 8474, show primer details
   
Sequence Name 29-K104830-0022-245-F03C-BA30
GenBank Accession LN499277 [GenBank]
Sequencing primer gene specific primer, BA30: 5'-ACTCAACTCTAACTCCGCATTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104830-0022-245-F03C-BA30
CTTCACTGTCTCATCAATGCATGGAGACATGCCTCAGAAGGAGAGAGACGCCATCATGAA
TGAGTTTCGGTCAGGTGATAGTCGTGTTTTGATAACAACAGATGTATGGGCACGTGGGAT
TGATGTGCAACAAGTAAGTCATTACTTATCCGCTATATTTTGTCTAGATAACAATGCGCG
GAATAAATTCAATCTCTGTTATTGAGGTCTCAGATTATGTTCCATTCTCTTATGGTAAAG
GTGAATTGACATGACAATCTTTATTTAACAGGTTTCTCTTGTTATCAATTATGATCTCCC
CAACAACCGTGAGCTCTACATCCATCGTATTGGGCGGTCTGGTCGATTCGGGCGTAAGGT
AAGAAACTTTTTCTAATCTCTGCTTCTGAGCTTAATTTCTTAGCCTGGAGATACTAGATG
GATATCTTTTAACCAATTTTGCGGTTATCTTTCTAGGGTGTTGCAATCAACTTTGTTAAA
AGCGACGACATCAAGATTCTCAGAGACATTGAGCAATGAGTATCTACATGAATGGCTTAT
CCATGAGTATATATTCATCAATATATTCAATCATGAGAATATATTCATTGGCATAAGATT
CTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCA
   
Sequence Name 44-K104831-0022-245-F03C-8409
GenBank Accession LN505177 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104831-0022-245-F03C-8409
GCCATTTACAATTGAATATATCCTGAGAATCTTATGCCAATGAATATATTCTCATGATTG
AATATATTGATGAATATATACTCATGGATAAGCCATTCATGTAGATACTCATTGCTCAAT
GTCTCTGAGAATCTTGATGTCGTCGCTTTTAACAAAGTTGATTGCAACACCCTAGAAAGA
TAACCGCAAAATTGGTTAAAAGATATCCATCTAGTATCTCCAGGCTAAGAAATTAAGCTC
AGAAGCAGAGATTAGAAAAAGTTTCTTACCTTACGCCCGAATCGACCAGACCGCCCAATA
CGATGGATGTAGAGCTCACGGTTGTTGGGGAGATCATAATTGATAACAAGAGAAACCTGT
TAAATAAAGATTGTCATGTCAATTCACCTTTACCATAAGAGAATGGAACATAATCTGAGA
CCTCAATAACAGAGATTGAATTTATTCCGCGCATTGTTATCTAGACAAAATATAGCGGAT
AAGTAATGACTTACTTGTTGCACATCAATCCCACGTGCCCATACATCTGTTGTTATCAAA
ACACGACTATCACCTGACCGAAACTCATTCATGATGGCGTCTCTCTCCTTCTGAGGCATG
TCTCCATGCATTGATGAGACAGTGAAGTTGTGACTTCTCATTTTCTCACTAAGATAATCC
ACCTATAACAAATGCGGAGTTGAAGTTGAG
Primers used for PCR BA31 - 8474, show primer details
   
Sequence Name 45-K118809-0022-245-F03C-BA31
GenBank Accession LN505695 [GenBank]
Sequencing primer gene specific primer, BA31: 5'-AATTTCATCAACTTGCTTTGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118809-0022-245-F03C-BA31
TCCAGCTTTACGAATAGATAACAATGAACCGTTTCAACTGTCGAGCAAAAGTCTTACAAG
TAGTGATAGTTGAGAGTTTTGACCCACAAACCACAATCAAAGCCCCCAGAATCAATTACG
AGGAAACGAAAAACCAAACGGTCCAAACCCTAAACCTAAATCGCTTCTCAAGTCATTAAA
CATTTACCACACGCGAGTTTAAGTTTGTTCAAAAGAGCCCACAGAAACATCAAAGGAAAT
ACACCGTTAAGATAGTGCAATAATTTGACAGAAGCGCTCAACAATACTTTTTCATCCAGA
CACTTCCCCGCTGAAAGCACAAAAATCTCAGACAAGCTCTTTTAGATAAGATCAGCTACA
TTCATTGGCATCTCATCAAAGTTGAGAATATATTCAACACTGAGAAATCTTGATGTCCGT
ATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 13-K118808-0022-245-F03C-8409
GenBank Accession LN492269 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118808-0022-245-F03C-8409
GCCATTTACATTGAATATATCCTGAATACGGACATCAAGATTTCTCAGTGTTGAATATAT
TCTCAACTTTGATGAGATGCCAATGAATGTAGCTGATCTTATCTAAAAGAGCTTGTCTGA
GATTTTTGTGCTTTCAGCGGGGAAGTGTCTGGATGAAAAAGTATTGTTGAGCGCTTCTGT
CAAATTATTGCACTATCTTAACGGTGTATTTCCTTTGATGTTTCTGTGGGCTCTTTTGAA
CAAACTTAAACTCGCGTGTGGTAAATGTTTAATGACTTGAGAAGCGATTTAGGTTTAGGG
TTTGGACCGTTTGGTTTTTCGTTTCCTCGTAATTGATTCTGGGGGCTTTGATTGTGGTTT
GTGGGTCAAAACTCTCAACTATCACTACTTGTAAGACTTTTGCTCGACAGTTGAAACGGT
TCATTGTTATCTTATTTCGTAAAGCTTGGATCATTGCAAAAATCAATCATTTGCAAAAGC
AAAGCAAGTTGATGAAAT

Last Updated on 06.12.2018 16:11