Sequences from confirmation amplicon


Confirmation sequences for line 248D08 and hit At2g17370 are shown.
Line ID 248D08
Locus At2g17370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7550173
Primers used for PCR PP08 - 8474, show primer details
   
Sequence Name 10-K102723-0022-248-D08C-8409
GenBank Accession LN490605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102723-0022-248-D08C-8409
GCCATTTACATTGAATAGAAGAAATAAACAGTAGCGAAGAAGAGCGAGAGGAAAAAAGTG
TTGGTTAGGTATAACGGAAGAGGAAGCGCGTCAGAGGCTTTACGAAGAGGAGGATCGACG
GCGACATTAGAGATTTCTTCTCCGTTCTTTTTAGTCGGAAATCTACGACGGAGATCCTCC
ATTGACGCCGGAGAGAAGGAGGAGATTTGTTTTTAATGGTTATGAGATTATTTTGGGCTC
CAAAGTCCAAAGTGTGAGATGACAAAAATAGGTGAAGAGTGTTATTTATAGTGTTGTGTG
TGACATCGAAATCAACGTCAAGGTTGTTGGGTCATCATCGTATGTGAGAGAGGAGAAGAA
GAAGAACAGAGATGATGATTTTGTCTCCTCCGTCACAATCTAACTGCTTCTTTTATTATT
TCCACTTTTGGGATGATTATTTAGGGTTATGGTTTTTGTTACTTTTGATTCTTTTTTTCA
ACACATTTAATGTTATGGATTTGATTGTTACTACTTACTAGTCCAACATGGATCAGTGAA
GAATTATTCACTTTTTAATAGTTATTCTGAAAATTAACCTAAAGATGAGGCGTTTATTTT
GGGTCTTTTTGAATCAAAAATTCAA
   
Sequence Name 26-K102728-0022-248-D08C-PP08
GenBank Accession LN497874 [GenBank]
Sequencing primer gene specific primer, PP08: 5'-GCATTGAAGGTTTGAATTTTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K102728-0022-248-D08C-PP08
CCTCATCTTAGGTAATTTTCAGAATAACTATTAAAAAGTGAATAATTCTTCACTGATCCA
TGTTGGACTAGTAAGTAGTAACAATCAAATCCATAACATTAAATGTGTTGAAAAAAAGAA
TCAAAAGTAACAAAAACCATAACCCTAAATAATCATCCCAAAAGTGGAAATAATAAAAGA
AGCAGTTAGATTGTGACGGAGGAGACAAAATCATCATCTCTGTTCTTCTTCTTCTCCTCT
CTCACATACGATGATGACCCAACAACCTTGACGTTGATTTCGATGTCACACACAACACTA
TAAATAACACTCTTCACCTATTTTTGTCATCTCACACTTTGGACTTTGGAGCCCAAAATA
ATCTCATAACCATTAAAAACAAATCTCCTCCTTCTCTCCGGCGTCAATGGAGGATCTCCG
TCGTAGATTTCCGACTAAAAAGAACGGAGAAGAAATCTCTAATGTCGCCGTCGATCCTCC
TCTTCGTAAAGCCTCTGACGCGCTTCCTCTTCCGTTATACCTAACCAACACTTTTTTCCT
CTCGCTCTTCTTCGCTACTGTTTATTTTCTTCTTATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11