Sequences from confirmation amplicon


Confirmation sequences for line 249D03 and hit At5g51870 are shown.
Line ID 249D03
Locus At5g51870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21088013
Primers used for PCR TN01 - 8474, show primer details
   
Sequence Name 23-K025043-022-249-D03C-8409
GenBank Accession LN496404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K025043-022-249-D03C-8409
AGATTTCCCGGACATGAAGCCATTTCTTGATTTTGTCTGAGTGAAAATTTCCTTCCGGAT
TTAGTTAGGGTTTCAATCTGATGAATCTTGGAATGAATATCGCAGGAACAATGGTGAGAG
GGAAGATCGAGATCAAGAAGATTGAGAACGTGACGAGCAGACAAGTCACGTTCTCGAAGC
GAAGAAGCGGTCTATTTAAGAAGGCTCATGAGCTTTCGGTTCTATGCGATGCTCAAGTAG
CCGCCATTGTCTTTTCTCAGAGCGGAAGATTACACGAATACTCTAGCTCCCAGTAAGATA
TTCTTTCATCAAATTATTTCAAAATATTTACACATGTCTATTTCCAAAATCTTTCTCTAG
CTACACATAGATTTGTTGAGTATCTAGAACTTCTGTAGTGAATAGTATAATATATCTCTG
GTTAAATCGTACTAGAGATTTTGCAATAATATAGGCGCTAAAGATTTGGCCCCCTTTCTT
TTGGTGCTAGCTAGTTCCTGACTTTGCCCAACAATGCCTTGTCACATTCTAATTTTAGAA
TTTAAGTTTTGTAAGTACTTAGCTTCTGGATTTTATTTAAGCAAACTTAAGAATTTTTAC
TACTTGAGAGAGTTTCTTCAAGCACGAGCAAGTGGATTGTATTAGGGTTTTCAGAAAAAA
AGTTTCTTGAACA
   
Sequence Name 24-K025043-022-249-D03C-TN01
GenBank Accession LN496844 [GenBank]
Sequencing primer gene specific primer, TN01: 5'-GGGTGTGTGGGTAACAGTTTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K025043-022-249-D03C-TN01
CAAGGAATACGAGGTTCACTGTTCAAGAAACTTTTTTTCTGAAAACCCTAATACAATCCA
CTTGCTCGTGCTTGAAGAAACTCTCTTAAGTAGTAAAAATTCTTAAGTTTGCTTAAATAA
AATCCAGAAGCTAAGTACTTACAAAACTTAAATTCTAAAATTAGAATGTGACAAGGCATT
GTTGGGCAAAGTCAGGAACTAGCTAGCACCAAAAGAAAGGGGGCCAAATCTTTAGCGCCT
ATATTATTGCAAAATCTCTAGTACGATTTAACCAGAGATATATTATACTATTCACTACAG
AAGTTCTAGATACTCAACAAATCTATGTGTAGCTAGAGAAAGATTTTGGAAATAGACATG
TGTAAATATTTTGAAATAATTTGATGAAAGAATATCTTACTGGGAGCTAGAGTATTCGTG
TAATCTTCCGCTCTGAGAAAAGACAATGGCGGCTACTTGAGCATCGCATAGAACCGAAAG
CTCATGAGCCTTCTTAAATAGACCGCTTCTTCGCTTCGAGAACGTGACTTGTCTGCTCGT
CACGTTCTCAATCTTCTTGATCTCGATCTTCCCTCTCACCATTGTTCCTGCGATATTCAN
TTCCAGATTCATCAGATTGAAACCCTAACTAAATCCGGAAGGAAATTTTCACTCAGACAA
AATCAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATA

Last Updated on 06.12.2018 16:11