Sequences from confirmation amplicon


Confirmation sequences for line 250C01 and hit At5g16080 are shown.
Line ID 250C01
Locus At5g16080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5252485
Primers used for PCR KF32 - 8474, show primer details
   
Sequence Name 44-K103355-0022-250-C01C-KF32
GenBank Accession LN505150 [GenBank]
Sequencing primer gene specific primer, KF32: 5'-AACAACAACATCACACCAAGTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103355-0022-250-C01C-KF32
GCGTCTGCTCTGCCCGTGGAGCGAGTAGAGACCATCCATGGTGTAACCCTCTGATGAGCT
CGGCGGGAGCGAAGTTACCGACGACGATGGTGTTCATGGCGGAGTTTGATATACTAAAAG
AACGAAACTTGGAGATGTGTAAAGTGATGAGAAGCCATGGGAAGAGAGTTGAAGGTATAG
TTCATGGAGGTGTTGGTCACGCTTTTCATATTCTCGACAACTCGTCGGTGTCACGTGATC
GAATCCACGACATGATGTGTCGTCTTCACAACTTCATACATCCATCATAATAATTCCCTA
AAGACCTTTTTCTGAAACCTTTAATTGATTTAAAATGTTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 60-K103356-0022-250-C01C-8409
GenBank Accession LN510233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103356-0022-250-C01C-8409
GCCATTTCACATTTTAAATCAATTAAAGGTTTCAGAAAAAGGTCTTTAGGGAATTATTAT
GATGGATGTATGAAGTTGTGAAGACGACACATCATGTCGTGGATTCGATCACGTGACACC
GACGAGTTGTCGAGAATATGAAAAGCGTGACCAACACCTCCATGAACTATACCTTCAACT
CTCTTCCCATGGCTTCTCATCACTTTACACATCTCCAAGTTTCGTTCTTTTAGTATATCA
AACTCCGCCATGAACACCATCGTCGTCGGTAACTTCGCTCCCGCCGAGCTCATCAGAGGG
TTACACCATGGATGGTCTCTACTCGCTCCACGTGGCAGAGCTAGACGCCAATATGCGTCG
GAAGCTGAAAGAGTAAGCGCCGATGACTTGGTGTGATGTTGTTGT

Last Updated on 06.12.2018 16:11