Sequences from confirmation amplicon


Confirmation sequences for line 251D07 and hit At5g57710 are shown.
Line ID 251D07
Locus At5g57710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23388153
Primers used for PCR PA53 - 8474, show primer details
   
Sequence Name 81-K037023-022-251-D07C-8409
GenBank Accession LN514024 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K037023-022-251-D07C-8409
CATTTACATTGAATATATCCATGTGGACACATTATAACAGTTACGGTTAAAAATAGTAAA
AAAAAAGGTGTTTATAGTTCTTTACTTCTTTTTGTTTTACTCCACAGTCTAGATTTTTGT
TGTGTTTTATGTATGGTAACGGGAGGGGTGATTTATGTAGCGATTATAGATATATTTATT
TGATAGATAAATAATGAAAGATTAATAAAGTTAAAAGTATGTTTTTGTTTTCTCAGTTAC
AAGATTAATCTAAAGAAGTTCAAACAATTGCATTTTCTTTAGTCGTCGTGTATATTTGGG
GACCATTTGATTTAAGAAGAAGTGTAAAAGCAGTTTTTAATGCAAGAAAGAAA
   
Sequence Name 82-K037023-022-251-D07C-PA53
GenBank Accession LN514146 [GenBank]
Sequencing primer gene specific primer, PA53: 5'-TTCTTTCTGCCATTAAAAACTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K037023-022-251-D07C-PA53
ATGGTCCCAAATATACACGACGACTAAAGAAATGCAATTGTTTGAACTTCCCTTTAAAAT
CTTGTAACTGAGAAAACAAAAACATACTTTCCTTTAAATTAATCTTTCATTATTTATCTA
TCAAATAAATATATCTATAATCGCTACATAAATCACCCCTCCCGTTACCATACATAAAAC
ACAACAAAAATCTAGACTGTGGAGTAAAACAAAAAGAAGTAAAGAACTATAAACACCTTT
TTTTTTACTATTTTTACCCCTAACTGTTATAATGTGTCCACATGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCATTAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
ACGTCCAAATGGG

Last Updated on 06.12.2018 16:11