Sequences from confirmation amplicon


Confirmation sequences for line 253F04 and hit At4g12360 are shown.
Line ID 253F04
Locus At4g12360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7328598
Primers used for PCR KH64 - 8474, show primer details
   
Sequence Name 46-K103233-0022-253-F04C-8409
GenBank Accession LN505834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103233-0022-253-F04C-8409
TTGAATATATGATGTATTCTTTTTATCTATGATATAAACATATATATGAGACTTGTTTTA
AAAGAGATAAATGGCATTTAAGGCGACAACAACAACAATATTTGGCTATCTAATGTTTTA
ATGCATGTATTCTACAAAAGTTATAACCAAAATGTTACCGGCAGTTTCGGAACACAAGTT
GGGATCAGTGGTGAGTCCACAAGTTTTGGAGAAAAGAGCATTGAAGTCGGTGACATTGAA
AGGGATCAACAACATTGGACTTTTGATGAAACCGCAAAGACAAGATAGCTGAGTTGTCAA
CGCCGTCTCTACGGCTGTGCAGCATTCCTGCGACGGAGGTCCTCGCTGAGTAATTGCCGG
AAGACAAACAGCCAAACCAGCCGCACAGTTCATAGCTTCGTTGACTGAAGGACTAGGAGC
CAGCGGAATATGAGGTCCCTGTCCTGAAACAGTCGTAGCTGCCACTAGCAATGTGGCTAG
GAGTAAGATCAAGG
   
Sequence Name 94-K103234-0022-253-F04C-KH64
GenBank Accession LN515303 [GenBank]
Sequencing primer gene specific primer, KH64: 5'-CCTTGATCTTACTCCTAGCCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K103234-0022-253-F04C-KH64
GACCTCATATTCCGCTGGCTCCTAGTCCTTCAGTCAACGAAGCTATGAACTGTGCGGCTG
GTTTGGCTGTTTGTCTTCCGGCAATTACTCAGCGAGGACCTCCGTCGCAGGAATGCTGCA
CAGCCGTAGAGACGGCGTTGACAACTCAGCTATCTTGTCTTTGCGGTTTCATCAAAAGTC
CAATGTTGTTGATCCCTTTCAATGTCACCGACTTCAATGCTCTTTTCTCCAAAACTTGTG
GACTCACCACTGATCCCAACTTGTGTTCCGAAACTGCCGGTAACATTTTGGTTATAACTT
TTGTAGAATACATGCATTAAAACATTAGATAGCCAAATATTGTTGTTGTTGTCGCCTTAA
ATGCCATTTATCTCTTTTAAAACAAGTCTCATATATATGTTTATATCATAGATAAAAAGA
ATACATCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11