Sequences from confirmation amplicon


Confirmation sequences for line 254G05 and hit At4g31360 are shown.
Line ID 254G05
Locus At4g31360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15221746
Primers used for PCR BL39 - 8474, show primer details
   
Sequence Name 08-K104736-0022-254-G05C-BL39
GenBank Accession LN489718 [GenBank]
Sequencing primer gene specific primer, BL39: 5'-AATTCTTCCTCTTAGCCTTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104736-0022-254-G05C-BL39
TTTGTTGAGTCTGACTTCGAGTTCGACTGTCCATGCTCTAAGTCACTCTAGACGACGCCA
ACGTCGTTAAAGGTTTCGCCTTTTCTTCTCCATCTGCCTTACTCTTCTTCGGCGGCATTG
TACGGACAGCAACAGGGTTTCGTCTTAACAATCTTCTTCTCCTTCTTCTCCGTCTCTGTC
TTACCGCTCTTTCTTACTTTTTTGTTGTTTGATATTCCCTTTTGTGTTTGCTTTTTTCTT
ACCTTGGAAAAGTAATTCGAATAAACGGTAGAGATTTAACCTACAAGGGTTATTAAAAGG
TTGACCGTTAGATTGCACGTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 24-K104737-0022-254-G05C-8409
GenBank Accession LN496979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104737-0022-254-G05C-8409
GCCTTTACATTGAATAATCCTACGTGCAATCTAACGGTCAACCTTCCTTTAACCCTTGTA
GGTTAAATCTCTACCGTTTATTCGAATTACTTTTCCAAGGTAAGAAAAAAGCAAACACAA
AAGGGAATATCAAACAACAAAAAAGTAAGAAAGAGCGGTAAGACAGAGACGGAGAAGAAG
GAGAAGAAGATTGTTAAGACGAAACCCTGTTGCTGTCCGTACAATGCCGCCGAAGAAGAG
TAAGGCAGATGGAGAAGAAAAGGCGAAACCTTTAACGACGTTGGCGTCGTCTAGAGTGAC
TCGGAGCATGGACAGTCGAACTCGAAGTCAGACTCAACAAAACGGTGCTAAAGCGGCTGG
ATCCGCCACTAAGCCGGCGACGAAGAAGGCTAAGAGGAAGAAT

Last Updated on 06.12.2018 16:11