Sequences from confirmation amplicon


Confirmation sequences for line 255F07 and hit At4g21323 are shown.
Line ID 255F07
Locus At4g21323 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11345914
Primers used for PCR CR11 - 8474, show primer details
   
Sequence Name 52-K017190-022-255-F07C-CR11
GenBank Accession LN507664 [GenBank]
Sequencing primer gene specific primer, CR11: 5'-GGAGCTAGCAACAACAAAATTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K017190-022-255-F07C-CR11
CGAACTATTAACAAGATCTCAAATTAGATACCTCGAAATTTAGGTTACGTTCGCAATTAT
TTCAATAACAAAAAGGAAAATAGAGAAAAAAGATAGAGAGGAAATAGAGAAGCTTAGGTT
AATGATGATCGACGACGAAGACGAATTAGCAACATTTGCAGTAATCTCTTTCGTGTCTTT
TCTGGAATTCGATGCTTCTTCATCAATTCGCAGTAACCTTCTTCAATTTTACCTATTTCA
TCAATTCGCTGTTTTTATTTAGCCATTTTTATTCTTTTCCATATTCGCCGAGTTGACAGA
CAGCCTATTTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 56-K017190-022-255-F07C-8409
GenBank Accession LN508962 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K017190-022-255-F07C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGAAATAGGCT
GTCTGTCAACTCGGCGAATATGGAAAAGAATAAAAATGGCTAAATAAAAACAGCGAATTG
ATGAAATAGGTAAAATTGAAGAAGGTTACTGCGAATTGATGAAGAAGCATCGAATTCCAG
AAAAGACACGAAAGAGATTACTGCAAATGTTGCTAATTCGTCTTCGTCGTCGATCATCAT
TAACCTAAGCTTCTCTATTTCCTCTCTATCTTTTTTCTCTATTTTCCTTTTTGTTATTGA
AATAATTGCGAACGTAACCTAAATTTCAAGGAATCTAATTTGAAATCTGGTTAATAGTCC
AAAATTAACCCCTAATTTTGTTG

Last Updated on 06.12.2018 16:11