Sequences from confirmation amplicon


Confirmation sequences for line 256E07 and hit At1g58060 are shown.
Line ID 256E07
Locus At1g58060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21495988
Primers used for PCR 27pv - 8474, show primer details
   
Sequence Name 27-K115234-0022-256-E07C-27pv
GenBank Accession LN498521 [GenBank]
Sequencing primer gene specific primer, 27pv: 5'-TGTAGTCATAATCTCGCCTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115234-0022-256-E07C-27pv
GGTTGCATCTGTTATGATAATCTGCTATTTTTTCACATATTCATTTTTGACACTACAATA
TGATGATGGCCTTATGATTTTCTGGCTTTCCCTTTAATAATGTTCCTTGGAGTTGTAGAT
TAAGCAAACTTAACTTGGCATATTTGTATCTTTGTTCAGGAGTGACAAGACTAGGCTGCT
GTTTTGTACTACTGGCATTCTACTGAGGAAACTCGCTGTAAGTCCTTCATTCTATCAATA
AGCTCTTGCAAGCAAATAATTCTTCTTACGTGTTATAGTGTTGTATGGTACTGAGAAAGA
GGAACATTGTTATGCTGCACAAACATTCTGGTGATGTGGACAAAATTGAAATATCATAGC
TGCATTACTGAAATTATGGTTTCTAGAGAACACGATTTTGCAGAAAGTATTATGTAGATA
GAGTCTCTGCTAAGTAAGGGGGACATTAGAAGTCCAATAGTTGACGTAGTTGGCTCATTA
TTAGTAGTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 03-K115233-0022-256-E07C-8409
GenBank Accession LN487609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115233-0022-256-E07C-8409
TGAGCCACTACGTCAACTATTGGACTTCTAATGTCCCCCTTACTTAGCAGAGACTCTATC
TACATAATACTTTCTGCAAAATCGTGTTCTCTAGAAACCATAATTTCAGTAATGCAGCTA
TGATATTTCAATTTTGTCCACATCACCAGAATGTTTGTGCAGCATAACAATGTTCCTCTT
TCTCAGTACCATACAACACTATAACACGTAAGAAGAATTATTTGCTTGCAAGAGCTTATT
GATAGAATGAAGGACTTACAGCGAGTTTCCTCAGTAGAATGCCAGTAGTACAAAACAGCA
GCCTAGTCTTGTCACTCCTGAACAAAGATACAAATATGCCAAGTTAAGTTTGCTTAATCT
ACAACTCCAAGGAACATTATTAAAGGGAAAGCCAGAAAATCATAAGGCCATCATCATATT
GTAGTGTCAAAAATGAATATGTGAAAAAATAGCAGATTATCATAACAGATGCAACCATAA
ATTAGTGAAGCCCAAAAAGAACAGCAAAACAAACAGGCGAGATTATGACTAC

Last Updated on 06.12.2018 16:11