Sequences from confirmation amplicon


Confirmation sequences for line 258B10 and hit At1g69810 are shown.
Line ID 258B10
Locus At1g69810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26278823
Primers used for PCR IS24 - 8474, show primer details
   
Sequence Name 25-K017279-022-258-B10C-IS24
GenBank Accession LN497225 [GenBank]
Sequencing primer gene specific primer, IS24: 5'-CTTTGGGTCCCTTCCTATAAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K017279-022-258-B10C-IS24
ACAATAATTTTTTTTTTGATGTAAAGAAGAAAAACAAAAAGAGCAGATCCAGTTGATCTC
AAGATCGCATCTGAATCATATAATAGCATGATCAAAGAGGAGACCGTTTCATACTTCCAA
ACTTTCGATGGTGTTATGGCAGAATCTGACAAGGTAAAACAAAACACTATTTCTCGATTT
AACCGTTTAAACCACAAATTTCTCTAATTCTTGTTTTATTTTCAATCTCCTTCTGGTGTT
TCAATCGGTTAGAATTAATCTCATGATCATATGATTCTGTTATATATTTACATATAGTTA
CCATATAATTCTTATGTCTGAAATTAATACAGTTTTACTGCATCTGATAAAAGTCAACTT
GCTTGTTCGGAAATGGTCTCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 26-K017279-022-258-B10C-8409
GenBank Accession LN497700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K017279-022-258-B10C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCCGAGACCATTTCCGAACAA
GCAAGTTGACTTTTATCAGATGCAGTAAAACTGTATTAATTTCAGACATAAGAATTATAT
GGTAACTATATGTAAATATATAACAGAATCATATGATCATGAGATTAATTCTAACCGATT
GAAACACCAGAAGGAGATTGAAAATAAAACAAGAATTAGAGAAATTTGTGGTTTAAACGG
TTAAATCGAGAAATAGTGTTTTGTTTTACCTTGTCAGATTCTGCCATAACACCATCGAAA
GTTTGGAAGTATGAAACGGTCTCCTCTTTGATCATGCTATTATATGATTCAGATGCGATC
TTGAGATCAACTGGATCTGCTCTTTTTGTTTTTCTTCTTTACATCAAAAAAAAAATTATT
GTCGTTTTCTTTTGCCTAACTAGGTTTTTATAGGAAGGGACCCAAA

Last Updated on 06.12.2018 16:11