Sequences from confirmation amplicon


Confirmation sequences for line 262F03 and hit At5g23430 are shown.
Line ID 262F03
Locus At5g23430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7898389
Primers used for PCR 41qc - 8474, show primer details
   
Sequence Name 22-K115806-0022-262-F03C-41qc
GenBank Accession LN496255 [GenBank]
Sequencing primer gene specific primer, 41qc: 5'-ATGACCAAAGAAATGAAAAATACT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115806-0022-262-F03C-41qc
CTTCGATCTAATCACAGAGCTAAAAGATAAGATGGCCACAAGAGTTTCAATTACATAAAA
AGAAGATGACGCCATGACACAGGGATCTATATAACTGGTTGGGCTGGGCTGGTAACCGCA
ATACAGTTGTGATCAGACACTCTTAGCCTAGAAGTTTTATTTTGTGTTGGTGTTAGAAAT
TTTTAAGAGTAATACAAACCTGTTGCCAGTAGGAATTCATGAGGATGAAAATCTAGGCTT
TGAATTTGCCCTTCATGACTCTTAAACTCGGTCAGCAGTTTTCCAGCTGTTAAATCCCAA
ACCTGGTAAAGACAAGCTGCATATCAGATCCATTAACACACAGAAAGATAAAAAAAAAGG
TTCCCGGTAAATTGCTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTAT
   
Sequence Name 01-K115805-0022-262-F03C-8409
GenBank Accession LN486711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115805-0022-262-F03C-8409
TCATTGAATATATCCTGAAAGCAATTTACCGGGAACCTTTTTTTTTATCTTTCTGTGTGT
TAATGGATCTGATATGCAGCTTGTCTTTACCAGGTTTGGGATTTAACAGCTGGAAAACTG
CTGACCGAGTTTAAGAGTCATGAAGGGCAAATTCAAAGCCTAGATTTTCATCCTCATGAA
TTCCTACTGGCAACAGGTTTGTATTACTCTTAAAAATTTCTAACACCAACACAAAATAAA
ACTTCTAGGCTAAGAGTGTCTGATCACAACTGTATTGCGGTTACCAGCCCAGCCCAACCA
GTTATATAGATCCCTGTGTCATGGCGTCATCTTCTTTTTATGTAATTGAAACTCTGGTGG
CCATCTTATCTTTTTAGCTCTGTGATTTGAGATCTGGAAGTAAATATGCATCTAAAGTAT
TTTTCATTTCNTTGGTC

Last Updated on 06.12.2018 16:11