Sequences from confirmation amplicon


Confirmation sequences for line 262H10 and hit At4g23390 are shown.
Line ID 262H10
Locus At4g23390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12219692
Primers used for PCR 20qc - 8474, show primer details
   
Sequence Name 27-K116083-0022-262-H10C-20qc
GenBank Accession LN498541 [GenBank]
Sequencing primer gene specific primer, 20qc: 5'-AACTTATGTCATCTTGTCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116083-0022-262-H10C-20qc
TTTGGTCCTTTAGGTATAGTTGTGGGTTTCAACTGCATTTATATTTCAATCCATCCAAAC
ATTAATAAGAATTTTATTTATAGATATTTTTTCGTGGTTTGTAGGATAAACCTTAATAGA
ATGGTTCTTGAGCAAAGGATGATCAAAAGCTAGCTGTTTTTGAATGTCGATGCAGTCAAA
TATATAACCATGTTCAGTCTGTCGAAATCAAAAGCAAGAAAAAATTGGTGTGGTCTGAAA
ATAAATGTAACTTGATGATAGTTGTAATATTGTTACAATAGTCAACAATTTTTGTAATGT
TTTATGAAAATTTAAGAAAAGAAAGGCTAAATTTACTTGGAAACTTTTGACTGCAGGTTT
ATTGAGATAGGTAAGTAGCTTCTTCATCTCTTCTTTCTCCTCTTTCGAAGGTATTGTTTT
GTGACTCTCTCCAACTAAAATGAGAGCTATTGTTGATAAAGTTAGCAAGATAATTAGTTT
ATTGGAATACTTCATCATGAGGATCAGCAATGAGTATTCAAGATAATTTCCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA
   
Sequence Name 11-K116082-0022-262-H10C-8409
GenBank Accession LN491271 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116082-0022-262-H10C-8409
CATTTACATTGAATATATCCTGGGAATTATCTTGAATACTCATTGCTGATCCTCATGATG
AAGTATTCCAATAAACTAATTATCTTGCTAACTTTATCAACAATAGCTCTCATTTTAGTT
GGAGAGAGTCACAAAACAATACCTTCGAAAGAGGAGAAAGAAGAGATGAAGAAGCTACTT
ACCTATCTCAATAAACCTGCAGTCAAAAGTTTCCAAGTAAATTTAGCCTTTCTTTTCTTA
AATTTTCATAAAACATTACAAAAATTGTTGACTATTGTAACAATATTACAACTATCATCA
AGTTACATTTATTTTCAGACCACACCAATTTTTTCTTGCTTTTGATTTCGACAGACTGAA
CATGGTTATATATTTGACTGCATCGACATTCAAAAACAGCTAGCTTTTGATCATCCTTTG
CTCAAGAACCATTCTATTAAGGTTTATCCTACAAACCACGAAAAAATATCTATAAATAAA
ATTCTTATTAATGTTTGGATGGATTGAAATATAAATGCAGTTGAAACCCACAACTATACC
TAAATGGACCAAAGATAACGACGCTTCTCATAAAACTAGTTCTCTGCCATTTCGACGAGA
GACGGTAAGTTAGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATATCCGCAC

Last Updated on 06.12.2018 16:11