Sequences from confirmation amplicon


Confirmation sequences for line 263B01 and hit At2g46270 are shown.
Line ID 263B01
Locus At2g46270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19000723
Primers used for PCR DR17 - 8474, show primer details
   
Sequence Name 04-K018976-022-263-B01C-DR17
GenBank Accession LN487728 [GenBank]
Sequencing primer gene specific primer, DR17: 5'-TAATATGCCTAGCAGCAACACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018976-022-263-B01C-DR17
AAATCATTATTACTCATGGTATTACTAGTACATAACTGGAGCGTAATAAAAAAAAGACTA
ACCTGCATAGCTGCCCAATCAGGGTAGACATGAACATTTGTTTGATCCTATCAACAAGAG
TCAAGTCAACGCATAAGGAATTTAAGAAAAACTAAATTGACTTTGGAATTATGGTTTGAA
ACTGTTCAACATACCACCGGGGGTGAAGATGGTTTATCTGATTTGGTAGGAGGCTTTGGT
TCCTCGCTGCTATTTCCCATAGGAATGGTTTCAAGGTTTCCCACTCAAGAGAACAATGAC
TCTTTCTGCAAAATCATGGGGATGGGTGTTGTTGTGTGAGAAATATAGAGACAGAGAAGG
GTAAAATAGTATTTCTCTCTCACATCCTATGTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 08-K018976-022-263-B01C-8409
GenBank Accession LN489522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018976-022-263-B01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACATAGGATGT
GAGAGAGAAATACTATTTTACCCTTCTCTGTCTCTATATTTCTCACACAACAACACCCAT
CCCCATGATTTTGCAGAAAGAGTCATTGTTCTCTTGAGTGGGAAACCTTGAAACCATTCC
TATGGGAAATAGCAGCGAGGAACCAAAGCCTCCTACCAAATCAGATAAACCATCTTCACC
CCCGGTGGTATGTTGAACAGTTTCAAACCATAATTCCAAAGTCAATTTAGTTTTTCTTAA
ATTCCTTATGCGTTGACTTGACTCTTGTTGATAGGATCAAACAAATGTTCATGTCTACCC
TGATTGGGCAGCTATGCAGGTTAGTCTTTTTTTTATTACGCTCCAGTTATGTACTAGTAA
TACCATGATTAATAATGATTTTCATGATGTGTTGCTGCT

Last Updated on 06.12.2018 16:11