Sequences from confirmation amplicon


Confirmation sequences for line 263F11 and hit At4g12020 are shown.
Line ID 263F11
Locus At4g12020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7201954
Primers used for PCR IS18 - 8474, show primer details
   
Sequence Name 31-K017280-022-263-F11C-IS18
GenBank Accession LN499944 [GenBank]
Sequencing primer gene specific primer, IS18: 5'-CGCTTTTAGTGCAGTTCTCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K017280-022-263-F11C-IS18
GAGCATGAGACAGAGTCCTGATCTTCCCCTAGCAAATCCTGTGCAATCTTCATGGCTGCA
TTTTTTACCACCACCATGGGCTCGGCAGAATGGCAAAGGACCACTAGCGCTTTTTCCGCA
GCCGTATGTTTTGCATCTCGCTCCTCCACCGTGCTTGACACAGAATTCTGTTCTTCCCCA
AGCAGATCGTGTGCAATCTTCATGGTTGCATCTTCGACCACCTCCATGAGCTATACAAAA
ATCAGTACTGCCTTCTGCGCCTTTGGTGCATCCAAGATATTCACATCTGCGACCACCTCC
GTGGGCTTTACAGTACACTGTTTTACCTTCAGCTCCCTTCTGGCAATCAGGTTTCTGGCA
TCTACGTCCACCGCCATGGGAAATGCAACGACCAGATGCATCTCTTGCTCCTTTTTGACA
TCCTTCCACTTGACATAACTTGGAACTAGAAGAATTATTAAGCTGCTGAGATAAACCGGA
ACCTGAGCTTATTGGTGGTGTTGCTGCCATGCTAGATGACAGTTCCAGTACACAAGCAGC
ATCAAGAACACAATTTTTTTTATATTGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATT
   
Sequence Name 32-K017280-022-263-F11C-8409
GenBank Accession LN500371 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K017280-022-263-F11C-8409
TAGATTCTCGGACATGAAGCCATTTACAATTGAATATATCCTGCAATATAAAAAAAATTG
TGTTCTTGATGCTGCTTGTGTACTGGAACTGTCATCTAGCATGGCAGCAACACCACCAAT
AAGCTCAGGTTCCGGTTTATCTCAGCAGCTTAATAATTCTTCTAGTTCCAAGTTATGTCA
AGTGGAAGGATGTCAAAAAGGAGCAAGAGATGCATCTGGTCGTTGCATTTCCCATGGCGG
TGGACGTAGATGCCAGAAACCTGATTGCCAGAAGGGAGCTGAAGGTAAAACAGTGTACTG
TAAAGCCCACGGAGGTGGTCGCAGATGTGAATATCTTGGATGCACCAAAGGCGCAGAAGG
CAGTACTGATTTTTGTATAGCTCATGGAGGTGGTCGAAGATGCAACCATGAAGATTGCAC
ACGATCTGCTTGGGGAAGAACAGAATTCTGTGTCAAGCACGGTGGAGGAGCGAGATGCAA
AACATACGGCTGCGGAAAAAGCGCTAGTGGTCCTTTGCCATTCTGCCGAGCCCATGGTGG
TGGTAAAAAATGCAGCCATGAAGATTGCACAGGATTTGCTAGGGGAAGATCAGGACTCTG
TCTCATGCACGGTGGGGGAAAGAGATGCCAAAGAGAGAACT

Last Updated on 06.12.2018 16:11