Sequences from confirmation amplicon


Confirmation sequences for line 265A07 and hit At2g27880 are shown.
Line ID 265A07
Locus At2g27880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11872661
Primers used for PCR PH29 - 8474, show primer details
   
Sequence Name 41-K017546-022-265-A07C-8409
GenBank Accession LN503899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017546-022-265-A07C-8409
ATACATCATACTCTTTCGAGTCAAAAGGTAATGGACCAGCAGTATAGAGGCTTTTCCTTC
CATCATACGCTGGTGACTTCCCTCCCAAGTGAGAGTCTTTATAATTCTTAACCAGAAGTT
TCATCACGTTTCTGTTCACTGTCTTTGATATAACCTCAGGATTGATCGAAACCTATAACA
CAATAACAATAACTTTTAGCCAAAAAATCTCTATATGACTAAAACAAGTCACAACCGCAG
ATTCATCATGTCAAAACTCTCTCTAGGAACTTTGTGAAATACATCAGATCAACAATAAAA
AGGAACAAACAACAGTATATTCGTCGAATGAAAGCAAAAAAAAAAAAAAACCATGGTTGC
AAAAACTAACAATTTTTCAGCCAAAAATCTCCCTGAAAAAACACCTTCAA
   
Sequence Name 37-K017547-022-265-A07C-PH29
GenBank Accession LN502438 [GenBank]
Sequencing primer gene specific primer, PH29: 5'-CGTTTGTGTTCATCTGAAGTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017547-022-265-A07C-PH29
GAGATTTCTGACTGAAAATTTGTTAGTTTCTGCATCATTGGTTTTTTTTTTTTTTGGCTT
TCATTCAACAAAAATACGGGGGTTGGTCCCTTTTTATGGGGGATCGGAGGTTTTTCCCAA
AGTCCCAAAAAAGAGTTTTGACATGATGAATCGGCGGGGGGGACTTGTTTTAGCCATATA
AAAATTTTTGGGCTAAAAGTTATTGTTATTGGGTTAAAGGTTCCAACCAACCCGGGGGTT
ATTTCAAAAACGGGGAACAAAAACGGGATGAAACTTCGGGTTAAAAATTATAAAAACCCC
CCCTTGGGGGGGAAGCCCCCCCCGTTTGAGGGAAGGAAAACCCCCTATACGGCGGGCCCA
TTACCTTTTGACCCAAAAAAGTATGATGCCTCCTGGGACCCCCAAGGAGTATGAGGGCCA
ATTTGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCAATTCAAAAAGGAAAATGCCC
CCCCCGTTTTTATAAAAGGAAAGTCCTTTTTGATAAAACGACAAATTCCGACCCGCCGTT
TTTATGGGCGAAAGCAATAAACAAATTTTTCTAATTCGGAAATCTTTTTTTCAACGGGT
Primers used for PCR FQ19 - 8474, show primer details
   
Sequence Name 05-K035332-022-265-A07C-8409
GenBank Accession LN488256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K035332-022-265-A07C-8409
TGTTTTACTCTTTGTGATGTGTTTGTATCGCCTTGTTTTTCATAGTTTTTTTTGGCATTG
TGACTCCTTTTGGCAGGAAGGACAGACCGTTTAAAGTTGCTGTAAAGAATGTGACAAGCA
CTGATCTTTATCAGTTGCAACAGTTCCTTGATCGTAAGCAAAGAGAGGCTCCATATGATA
CTATCCAAGTGCTTGATGTTGTTCTTAGGGATAAGCCCTCTAATGAGTAAGAAATCTGAA
ATTTGCATAAACTTCAGTATTGTCAAAAGCTATGCATAAACTTAACATTTCCCTGTTTTG
CTTTCTGTTCAGTTATGTCTCTGTTGGGAGGT
   
Sequence Name 05-K035332-022-265-A07C-FQ19
GenBank Accession LN488257 [GenBank]
Sequencing primer gene specific primer, FQ19: 5'-AAAGACCTCCCAACAGAGACATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K035332-022-265-A07C-FQ19
GCACAGGGGATGTTAAGTTATGCATAGCTTTTGAAATACTGAAGTTTATGCAAATTTCAG
ATTTCTTACTCATTAGAGGGCTTATCCCTAAGAACAACATCAAGCACTTGGATAGTATCA
TATGGAGCCTCTCTTTGCTTACGATCAAGGAACTGTTGCAACTGATAAAGATCAGTGCTT
GTCACATTCTTTACAGCAACTTTAAACGGTCTGTCCTTCCTGCCAAAAGGAGTCACAATG
CCAAAAAAAACTATGAAAAACAAGGCGATACAAACACATTCACAAAGAGTTAAAACAAGA
CATTACCCAGAGGAACCGTCACAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGA

Last Updated on 06.12.2018 16:11