Sequences from confirmation amplicon


Confirmation sequences for line 267E12 and hit At1g15240 are shown.
Line ID 267E12
Locus At1g15240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5243746
Primers used for PCR KR50 - 8474, show primer details
   
Sequence Name 90-K100901-0022-267-E12C-8409
GenBank Accession LN515017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100901-0022-267-E12C-8409
GCCTTTACATTGAATATGACATGAAATTACCAGACAGAGCGGACGGATTTCTTCCAGAAC
TTAAGGACCCATTCCCGGGGAATTAGGACCCGATCTTCGAAAAACGTCCGGATCAGGAAA
GATGAGCTGGTTTAGAAACAATGAAAAGGGAAGAGAGGGAGAGAGAATCAATGGCTGGGT
AGTCAAATACGGTATACGAATTGATAGAATCGGCGGTAATGGATTCTTTCCCGGGGAAGG
TTGTTGATTCCGTACGTTTCTCCGGCGAATGTTCCCGGGAATACGGTAGGAGGATCTTTT
AATTCTCTCTCTTCGTTGTTGTTAGC
   
Sequence Name 90-K100907-0022-267-E12C-KR50
GenBank Accession LN515018 [GenBank]
Sequencing primer gene specific primer, KR50: 5'-TGCTAACAACAACGAAGAGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100907-0022-267-E12C-KR50
CGGGACATTCGCCGGAGAAACGTACGGAATCAACAACCTTCCCCGGGAAAGAATCCATTA
CCGCCGATTCTATCAATTCGTATACCGTATTTGACTACCCAGCCATTGATTCTCTCTCCC
TCTCTTCCCTTTTCATTGTTTCTAAACCAGCTCATCTTTCCTGATCCGGACGTTTTTCGA
AGATCGGGTCCTAATTCCCCGGGAATGGGTCCTTAAGTTCTGGAAGAAATCCGTCCGCTC
TGTCTGGTAATTTCATGTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCANAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11