Sequences from confirmation amplicon


Confirmation sequences for line 268A08 and hit At4g21320 are shown.
Line ID 268A08
Locus At4g21320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11341053
Primers used for PCR YC01 - 8474, show primer details
   
Sequence Name 51-K026964-022-268-A08C-8409
GenBank Accession LN507380 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K026964-022-268-A08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGTTCCTGAGGAAACTCTTCTACGA
TATGTACGTTTGATTAAGAACGGTGGTCTCAGAGCCAAACCAATGTTTGCTGTGAAATTC
AACAAGTCTGATATTCCTGGTAGGAATAGAGCATTTGGTTCCTATGTTGTTCCAGAACCT
CGGTCAAGCGGTAAGAAGAAACAACTAGGAGTTATTTGTTGGATTTGCAGTGAGAGTTTC
AGATTTATATGCTTACTGTGAGAATGCCTTTGTTTCACAGAGTTTGTGGAGGACATAGAT
CTCTTGATCAGAAAGGCTGAACGGTGTTTAGAAGCCGGGGCAGACACTATTATGATAGAT
GCGGATGATGTCTGCAAATACGCGGATTCCCTTCGAGCAGACATAATTGCTAAAGTCATA
GGACGTTTGGGAATAGAGAAGACTATGTTTGAAGCATCTGATGCGAAGTTGGTTGAGTGG
TTCATCAAACGTTATGGTCCAAATGTATTTGCTGATACTCTAGTCCTCTCTATTATTTTT
AACCTTCTGTTTGAATGGTCACCCTCACAAGATTCATATCTCTTCGTC
   
Sequence Name 55-K026964-022-268-A08C-YC01
GenBank Accession LN508689 [GenBank]
Sequencing primer gene specific primer, YC01: 5'-AGAGATTCACCTGAAAAACCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K026964-022-268-A08C-YC01
ATATGAATCTTGTGAGGGTGACCATTCAAACAGAAGGTTAAAAATAATAGAGAGGACTAG
AGTATCAGCAAATACATTTGGACCATAACGTTTGATGAACCACTCAACCAACTTCGCATC
AGATGCTTCAAACATAGTCTTCTCTATTCCCAAACGTCCTATGACTTTAGCAATTATGTC
TGCTCGAAGGGAATCCGCGTATTTGCAGACATCATCCGCATCTATCATAATAGTGTCTGC
CCCGGCTTCTAAACACCGTTCAGCCTTTCTGATCAAGAGATCTATGTCCTCCACAAACTC
TGTGAAACAAAGGCATTCTCACAGTAAGCATATAAATCTGAAACTCTCACTGCAAATCCA
ACAAATAACTCCTAGTTGTTTCTTCTTACCGCTTGACCGAGGTTCTGGAACAACATAGGA
ACCAAATGCTCTATTCCTA

Last Updated on 06.12.2018 16:11