Sequences from confirmation amplicon


Confirmation sequences for line 269D05 and hit At5g56740 are shown.
Line ID 269D05
Locus At5g56740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22955702
Primers used for PCR ER02 - 8474, show primer details
   
Sequence Name 05-K023017-022-269-D05C-8409
GenBank Accession LN488176 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K023017-022-269-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATACACACCACATATGACTTAGCAA
GGCCTGTTAACGGCCCAAATGCAAGACCAATTCAAGTTTGTATTATTAGGTATTATTTAT
CTAATTAACGAAATATCAAATGAGGGCGTTTGGTCTAGTGGTATGATTCTCGCTTTGGGT
GCGAGAGGTCCCGAGTTCGATTCTCGGAACGCCCCATTTGTATTTTTTATGTTCACACTC
TTTTTGCCACTCTTTTTGCCAAATAGTCCATTTTTCCCAAAAGATCCATAACTTTAAGAA
CATGTAATCATTAACCAAAGTTAGTTATTCTTGGTGAATCTAGTTGACTTTGGGAGAGGA
GAAAGAACCGATTTTAGTTATTCTTCCCCATTATTGAAGTCTAGTTCTCGGCCAAATATT
CAACAACAAAAAATTGACTATTGTGAAAATTAGGCTTGTTTTGAAATAGGTCTAACTTAA
GATATTAACCATAAATTCGGTTATTGTA
   
Sequence Name 06-K023017-022-269-D05C-ER02
GenBank Accession LN488624 [GenBank]
Sequencing primer gene specific primer, ER02: 5'-CCAAACAACAATACAATAACCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K023017-022-269-D05C-ER02
TTTATGGTTAATATCTTAAGTTAGACCTATTTCAAAACAAGCCTAATTTTCACAATAGTC
AATTTTTTGTTGTTGAATATTTGGCCGAGAACTAGACTTCAATAATGGGGAAGAATAACT
AAAATCGGTTCTTTCTCCTCTCCCAAAGTCAACTAGATTCACCAAGAATAACTAACTTTG
GTTAATGATTACATGTTCTTAAAGTTATGGATCTTTTGGGAAAAATGGACTATTTGGCAA
AAAGAGTGGCAAAAAGAGTGTGAACATAAAAAATACAAATGGGGCGTTCCGAGAATCGAA
CTCGGGACCTCTCGCACCCAAAGCGAGAATCATACCACTAGACCAAACGCCCTCATTTGA
TATTTCGTTAATTAGATAAATAATACCTAATAATACAAACTTGAATTGGTCTTGCATTTG
GGCCGTTAACAGGCCTTGCTAAGTCATATGTGGTGTGTATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR FQ11 - 8474, show primer details
   
Sequence Name 12-K035333-022-269-D05C-8409
GenBank Accession LN491478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K035333-022-269-D05C-8409
TTCCGGACATGAGCCATTTACATAAAGAGAGCCACAAGCTTACTGACTCTCTTGTGTATC
GCTCTCTCCGCATTTCCATTAACCCTCCAATTTTCCCGCCTTTGTCTCACTCTCTTCCCG
CCTTGCACTCGCCGGGGACTCCATGGTTCAGAAGCAGCAAGCCTCCGCCGGTCCGGGTAC
CGAGCCTAAGAAGCGTCGCCGTGTCGGATTTTCTCCCGCCGGTGAGTCATTTCTTCTTCT
TGTTTGTTTTCCTTTACTCGCGATTACCACTTTCCCAGCTCCGATTTCGTATTTTTCTTT
AATCCGTTGATTTTTCTTATGTGCAGATACTGGTGTCGAGGCTAACGAGTGCATCAAAAT
TTATCTCGGTGCGT
   
Sequence Name 12-K035333-022-269-D05C-FQ11
GenBank Accession LN491479 [GenBank]
Sequencing primer gene specific primer, FQ11: 5'-AAATACGCACCGAGATAAATTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K035333-022-269-D05C-FQ11
ATCTGCACATAAGAAAATCAACGGATTAAAGAAAATACGAAATCGGAGCTGGGAAAGTGG
TAATCGCGAGTAAAGGAAAACAAACAAGAAGAAGAAATGACTCACCGGCGGGAGAAAATC
CGACACGGCGACGCTTCTTAGGCTCGGTACCCGGACCGGCGGAGGCTTGCTGCTTCTGAA
CCATGGAGTCCCCGGCGAGTGCAAGGCGGGAAGAGAGTGAGACAAAGGCGGGAAAATTGG
AGGGTTAATGGAAATGCGGAGAGAGCGATACACAAGAGAGTCAGTAAGCTTGTGGCTCTC
TTTATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCACGTCCCAATG

Last Updated on 06.12.2018 16:11