Sequences from confirmation amplicon


Confirmation sequences for line 270C03 and hit At3g48890 are shown.
Line ID 270C03
Locus At3g48890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18130508
Primers used for PCR UF24 - 8474, show primer details
   
Sequence Name 19-K030219-022-270-C03C-8409
GenBank Accession LN494637 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K030219-022-270-C03C-8409
ATTGAATATATTGCTATATTATAATATCATATTGATTAATTATCCACATTTGCTTGCTAT
TTGTGTTCCATGACGTTTGTAAATTAAGTTTTGGCGAACATTGTTGGTATGTAACCATGC
TGCAGTTGTCTAGATCCCTGACTTGTCAACTAGACAGTTCTTTTACATTCCAGTTACCGA
AAACTTCGTTGTGGTAATTAGGTTTTGTGCTAGATGAAATAATCACTTCAGTGATCATAC
CGTTTTGCTTGTTCATAGTGGCGACAGACACAGACACACTCATCAACGCATATACATGTC
GATGTATATGTATGCATAACTTTTAGTTTTTTTCTTGTACATAGTTCATCAGCTGTACTA
CATATCTAGATGAATCATATTCATATGTGCCAAGCTGTAGTTTTTCATTCTTCAAACTCT
ACTTAACTCTTACTGGTCGCTAATTTGCATGACCATCTGTTCTTTNTTATGC
   
Sequence Name 20-K030219-022-270-C03C-UF24
GenBank Accession LN495091 [GenBank]
Sequencing primer gene specific primer, UF24: 5'-CTGCATAAAAAGAACAGATGGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K030219-022-270-C03C-UF24
AGTAGAGTTTGAAGAATGAAAAACTACANGCTTGGCACATATGAATATGATTCATCTAGA
TATGTAGTACAGCTGATGAACTATGTACAAGAAAAAAACTAAAAGTTATGCATACATATA
CATCGACATGTATATGCGTTGATGAGTGTGTCTGTGTCTGTCGCCACTATGAACAAGCAA
AACGGTATGATCACTGAAGTGATTATTTCATCTAGCACAAAACCTAATTACCACAACGAA
GTTTTCGGTAACTGGAATGTAAAAGAACTGTCTAGTTGACAAGTCAGGGATCTAGACAAC
TGCAGCATGGTTACATACCAACAATGTTCGCCAAAACTTAATTTACAAACGTCATGGAAC
ACAAATAGCAAGCAAATGTGGATAATTAATCAATATGATATTATAATATAGCAATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACA
Primers used for PCR 04am - 3144, show primer details
   
Sequence Name 01-K106680-0022-270-C03C-3144
GenBank Accession LN486589 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106680-0022-270-C03C-3144
ATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTA
CCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTCGTCATGTATATTGAATATTTTCTTT
CGCCACAATAGCAATATTAGAAGCCAAACCTTTGACGAGTTTGTACGTTATCAATCTCTA
CTAATCCAAGATGTAATCTAAATACAGGTTTTTCTAGTATAAGAAAAAGTAGAGATCTTT
CCGATAACTTGAATACAATACAATTGTATCAGTGCTTCTAATGGTATTGTTCCAAGAAAA
GAAGTGAAAAAACAAACACTAAGATGCAAAAAGCAATAAAAAGAGCATCATAAGATTATG
AACAAAAAGGAGAGAATATTCATCAAAAAGATTTACTAAAAGTGTTCAGTTTTACAATAA
AGAATCACTTAAACTAGAGTTCGAGCATCAGTAGATATACTAGAATCATAAGATAGATAG
TAATTGGAAACTTCGGATTATAAATCAAACAGTCAACCATGATTAACTAACCTAAACCCC
AAATCTCGAAACTATTGGGTTGAATCGTTTATTACTGGTAATTTAACTAGTGATCATTTG
GATTCAATCATAATTTAATGAACAAGTAAAGATTTCACAAGAAATA
   
Sequence Name 17-K106681-0022-270-C03C-04am
GenBank Accession LN493868 [GenBank]
Sequencing primer gene specific primer, 04am: 5'-TCTCAGAGCAGGTATTTCTTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106681-0022-270-C03C-04am
CTAATATGATTGATCCAATGATCACTAGTTAAATTACCAGTAATAAACGATTCAACCCAA
TAGTTTCGAGATTTGGGGTTTAGGTTAGTTAATCATGGTTGACTGTTTGATTTATAATCC
GAAGTTTCCAATTACTATCTATCTTATGATTCTAGTATATCTACTGATGCTCGAACTCTA
GTTTAAGTGATTCTTTATTGTAAAACTGAACACTTTTAGTAAATCTTTTTGATGAATATT
CTCTCCTTTTTGTTCATAATCTTATGATGCTCTTTTTATTGCTTTTTGCATCTTAGTGTT
TGTTTTTTCACTTCTTTTCTTGGAACAATACCATTAGAAGCACTGATACAATTGTATTGT
ATTCAAGTTATCGGAAAGATCTCTACTTTTTCTTATACTAGAAAAACCTGTATTTAGATT
ACATCTTGGATTAGTAGAGATTGATAACGTACAAACTCGTCAAAGGTTTGGCTTCTAATA
TTGCTATTGTGGCGAAAGAAAATATTCAATATACATGACGACCGAAGGCGGGAAACGACA
ATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAG
GGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATTC

Last Updated on 06.12.2018 16:11