Sequences from confirmation amplicon


Confirmation sequences for line 270E04 and hit At4g35190 are shown.
Line ID 270E04
Locus At4g35190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16748111
Primers used for PCR PA58 - 8474, show primer details
   
Sequence Name 25-K037017-022-270-E04C-8409
GenBank Accession LN497352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K037017-022-270-E04C-8409
CCATTTCAATTGAATATATTCCTAAACTTTAAGAATTGATCAATGTTTAGGGATATAATT
ATGAGAAATATTAAAGTTTGTTGTTTTCTGGGGTTTCTTTTGTCCCTCTTGAAGAAACCA
CCTGTATCAAACTTTCAACAAACTGATTCCACCAAAATGCACAACCTGAAAATATCAGCA
CTGAATCTTGGCCAATTCTTTGTAGAAAATGAACACACAATCTCTTGTGCTGTATACCAA
AGAGTTCATTCCCTCAATTATGAAGATCAGAAAGAACAACACAAACCATCACAATGATAT
TTTGTGGTTGCAGCGGGATCCAACTTTCCCCAAATCTTACGGAGCAGGAATAGGCTGTTC
CATTGGGTCGGAGAAACTCTCTGCTCTGATGAATTTGATATATAAATATATAATTGATAC
TATATTTTTTGGTACCGAAAGACTTTTTAATTGAACCCGATAAATCAAACTTCAAA
   
Sequence Name 26-K037017-022-270-E04C-PA58
GenBank Accession LN497831 [GenBank]
Sequencing primer gene specific primer, PA58: 5'-TTTGAAGTTGATTTTATCGGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K037017-022-270-E04C-PA58
AAAAATATAGTATCAATTATATATTTATATATCAAATTCATCAGAGCAGAGAGTTTCTCC
GACCCAATGGAACAGCCTATTCCTGCTCCGTACGATTTGGGGAAAGTTGGATCCCGCTGC
AACCACAAAATATCATTGTGATGGTTTGTGTTGTTCTTTCTGATCTTCATAATTGAGGGA
ATGAACTCTTTGGTATACAGCACAAGAGATTGTGTGTTCATTTTCTACAAAGAATTGGCC
AAGATTCAGTGCTGATATTTTCAGGTTGTGCATTTTGGTGGAATCAGTTTGTTGAAAGTT
TGATACAGGTGGTTTCTTCAAGAGGGACAAAAGAAACCCCAGAAAACAACAAACTTTAAT
ATTTCTCATAATTATATCCCTAAACATTGATCAATTCTTAAAGTTTAGGAATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCA

Last Updated on 06.12.2018 16:11