Sequences from confirmation amplicon


Confirmation sequences for line 270H01 and hit At1g63910 are shown.
Line ID 270H01
Locus At1g63910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23721230
Primers used for PCR RS02 - 8474, show primer details
   
Sequence Name 31-K016848-022-270-H01C-8409
GenBank Accession LN499942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K016848-022-270-H01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTTTACTAACTCGACTTAAATTTTTATATATT
ATCAATTCAAGATAGTGTATTATTATAGACCGCTACAGGAAGTCTTATAAGATTTTCAAT
ACTGATACCATTTTCAATAATAAGTTTGTTGGGTTGGGATACATACATAGTACATTTTAT
ACTTATATTCAGTTTATCATGTTATATTTTAAATTTAAACAATTATATGTTTAGTTTGGC
CACGGTTTGAAGCAACGACTAGCCTACTGTCGATGACCAAAGAATCTTTAACGTGTAGTT
TCATTCATCTAGCTCGTTTACTTTTGTCTACTTGGTCGAGCTATTAAGTAGTACACTTTA
AGTTACTTCATTGAACATTCTCAAAATGTGATACATACATATTTTCTTATTAAACTTATG
TTTTTTGTTTTTTTCAGGTGGGCT
   
Sequence Name 32-K016848-022-270-H01C-RS02
GenBank Accession LN500369 [GenBank]
Sequencing primer gene specific primer, RS02: 5'-ACTAGCTATATGAGCCCACCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K016848-022-270-H01C-rs02
AAAAAACATAAGTTTAATAAGAAAATATGTATGTATCACATTTTGAGAATGTTCAATGAA
GTAACTTAAAGTGTACTACTTAATAGCTCGACCAAGTAGACAAAAGTAAACGAGCTAGAT
GAATGAAACTACACGTTAAAGATTCTTTGGTCATCGACAGTAGGCTAGTCGTTGCTTCAA
ACCGTGGCCAAACTAAACATATAATTGTTTAAATTTAAAATATAACATGATAAACTGAAT
ATAAGTATAAAATGTACTATGTATGTATCCCAACCCAACAAACTTATTATTGAAAATGGT
ATCAGTATTGAAAATCTTATAAGACTTCCTGTAGCGGTCTATAATAATACACTATCTTGA
ATTGATAATATATAAAAATTTAAGTCGAGTTAGTAAAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11