Sequences from confirmation amplicon


Confirmation sequences for line 274B08 and hit At1g06180 are shown.
Line ID 274B08
Locus At1g06180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1890281
Primers used for PCR GD03 - 8474, show primer details
   
Sequence Name 10-K021339-022-274-B08C-8409
GenBank Accession LN490457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K021339-022-274-B08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAATGAGTAACTTGAC
TGTAGATTTCACAATTTTCTCTTTGTATATGTGTTGCTTTTGTCAATATGCGAAATTTTA
TATCTTTGTGGGGCAGAGAACTGAGAAGATTCCAACCCGTGATTGCCTTTGTCCATTAAA
TAATTTCAATTTTTTACTTTCCTCTAATTATACTTTTTATTCACTAGTAGACAAAGATAG
GCACTGTGATCAGTGTAAGACATGTGCAAATTCTTTTAACCATACGCATGAACTTCAATA
ACTTTCCTTTTGTTTTACTCGCTCTTTGTTCTCATAACAATGTAGACGACGTATTATGAT
GATTGATGAGTCATTCATGTGACATTTGTTTTGAGTAGATGGTCTGCGATAGCTGCAAAA
TTGCCTGGACGAACAGACAACGAAATTAAAAATGTTTGGCACACTCATTTAAAGAAAAGA
CTCCACCACAGTCAAGATCAAAACAACAAGGAAGATTTCGTCTCTACTACAGCTGCGGAG
ATGCCAACCTCTCCGCAACAACAATCTAGTAGTAGTGCCGACATTTCAGCAATTACAACA
TTGGGAAACAACAATGACATCTCCAATAGCAACAAAGACTCCGCGACGTCATCCGAAGA
   
Sequence Name 11-K021339-022-274-B08C-GD03
GenBank Accession LN490918 [GenBank]
Sequencing primer gene specific primer, GD03: 5'-TATAATTGCAAGAACATCTTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K021339-022-274-B08C-GD03
TCTTTGTTGCTATTGGAGATGTCATTGTTGTTTCCCAATGTTGTAATTGCTGAAATGTCG
GCACTACTACTAGATTGTTGTTGCGGAGAGGTTGGCATCTCCGCAGCTGTAGTAGAGACG
AAATCTTCCTTGTTGTTTTGATCTTGACTGTGGTGGAGTCTTTTCTTTAAATGAGTGTGC
CAAACATTTTTAATTTCGTTGTCTGTTCGTCCAGGCAATTTTGCAGCTATCGCAGACCAT
CTACTCAAAACAAATGTCACATGAATGACTCATCAATCATCATAATACGTCGTCTACATT
GTTATGAGAACAAAGAGCGAGTAAAACAAAAGGAAAGTTATTGAAGTTCATGCGTATGGT
TAAAAGAATTTGCACATGTCTTACACTGATCACAGTGCCTATCTTTGTCTACTAGTGAAT
AAAAAGTATAATTAGAGGAAAGTAAAAAATTGAAATTATTTAATGGACAAAGGCAATCAC
GGGTTGGAATCTTCTCAGTTCTCTGCCCCACAAAGATATAAAATTTCGCATATTGACAAA
AGCAACACATATACAAAGAGAAAATTGTGAAATCTACAGTCAAGTTACTCATTACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGC

Last Updated on 06.12.2018 16:11