Sequences from confirmation amplicon


Confirmation sequences for line 274G12 and hit At1g73670 are shown.
Line ID 274G12
Locus At1g73670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27699960
Primers used for PCR DL56 - 8474, show primer details
   
Sequence Name 15-K018613-022-274-G12C-DL56
GenBank Accession LN492761 [GenBank]
Sequencing primer gene specific primer, DL56: 5'-CAGATTCCATCAACTCAAAGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018613-022-274-G12C-DL56
CGAAACTCTCTTCGAGAAGGAGGAAGCATAATATGTTTAATCTCAACAACATCTGGATGA
AGAAGCAAGCGTAACAACTTAATCTCCCTAAGAATCCTGGTTGCATCAGAGATATGATCA
AACACATCGTTAATCTTTTTGATAGCAACTCTTTCTCCAGTGTGAGTATCTATAGCAGAA
CCAACAACTCCATAGCTTCCTTTGCCAACTACTTCTTGAATCTGATATCTATTTGCTTCT
CCATACTCCGTGAAGAATTCAGCATTAGGTATTCCCTGAGAAATTGCTAGATTTTATTCA
TGATTTTTCTCGGAATGAATCAAATCTAGATATCAAAATTGGAAACAAAATCTACCTTCT
TCTCCATAGGTAAGTGATTTCGTTGCTGAACCTTAATCTGTCTTAGCTTAGACGGATCGG
TAAGCTTCTTCAAATCTTCGTCGTCGTTGGGATTAGAAACGGTGGGGGGATTTTGAATCT
GATCGTGATTATTGGAAGAAGAAGAAGAAGAAGGACGACGTTGAAAGAAAAGCCAACGAC
GAACACCGTCGACGAGATTGCCACCACCACCCATTGATTTAGAGGCTGATAATAATAAAT
TACAGATTTTGTTTTTCCGATTTTACTCTCAGCCAAGCGTTTCTTCGTCAAATCATTTCC
ACCTTATTCGCCGTTTCTCTTGCTGAAATATTTTTTT
   
Sequence Name 16-K018613-022-274-G12C-8409
GenBank Accession LN493231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018613-022-274-G12C-8409
AGATTTCCCGGACATGAAGCCATTTTTTCTCTGCGCGCGTCTCTGTCTGTCTCTTTCAGA
TTCAACTGGAGCAAGTACGAAACATAAATATCAATAAATTAAAGGAAAAAAAATAACAGT
AGAGAAGAAGAACATGTTTTCAACTCCTCGGGAGCAAAAAAAATAATTCAGCAAGAGAAA
CGGCGAATAAGGTGGAAATGATTTGACGAAGAAACGCTTGGCTGAGAGTAAAATCGGAAA
AACAAAATCTGTAATTTATTATTATCAGCCTCTAAATCAATGGGTGGTGGTGGCAATCTC
GTCGACGGTGTTCGTCGTTGGCTTTTCTTTCAACGTCGTCCTTCTTCTTCTTCTTCTTCC
AATAATCACGATCAGATTCAAAATCCCCCCACCGTTTCTAATCCCAACGACGACGAAGAT
TTGAAGAAGCTTACCGATCCGTCTAAGCTAAGACAGATTAAGGTTCAGCAACGAAATCAC
TTACCTATGGAGAAGAAGGTAGATTTTGTTTCCAATTTTGATATCTAGATTTGATTCATT
CCGAGAAAAATCATGAATAAAATCTAGCAATTTCTCAGGGAATACCTAATGCTGAATTCT
TCACGGAGTATGGAGAAGCAAATAGATATCAGATTCAAGAAGTAGTTGGCAAAGGAAGCT
ATGGAGTTGTTGGTTCTGCTATAGATAC

Last Updated on 06.12.2018 16:11