Sequences from confirmation amplicon


Confirmation sequences for line 276G04 and hit At3g57390 are shown.
Line ID 276G04
Locus At3g57390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21235795
Primers used for PCR SZ02 - 8474, show primer details
   
Sequence Name 34-K018601-022-276-G04C-SZ02
GenBank Accession LN501228 [GenBank]
Sequencing primer gene specific primer, SZ02: 5'-CACTCTTGTTTTGTTTCCTTGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K018601-022-276-G04C-SZ02
GCATTGGAAGAAAACCAAATCTTGCGCAAACAGGTAAAGATTCATCAAAACCCTTATATT
AGCTCGGTCTGGTCTTCACCAGTCATAACTGAGTTCAATTCTAATTTGCCCTCAGGTTGA
GATGTTGGGGAGAGGTTCAGGACCAAAAGTGTTGAATGAAAGGCCTCAAGATTCTAGCCC
AGAAGCCGATCCCGAGAGCTCTTCATCAGAAGAGGATGAGAATGACAACGAGGAGCACCA
TTCCGACACTTCCTTGCAGTTGGGGTAAACTGCAAAACAAAGCCCTAACTTCTGTTACAA
GCTTTTATTGTTACTGAGCATTCTACAAACTTGTTGGTTTGATCTTTCTTAGGTTGTCGT
CGACGGGGTATTGCACAAAGAGAAAGAAGCCGAAGATCGAACTGGTCTGCGATAACTCTG
GGAGTCAAGTGGCTTCTGATTGATGGAATCGATTATTTTTCTAATTCTGGTTGTTTAGGG
GTCTCTATGTGTCTTCTTGTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 35-K018601-022-276-G04C-8409
GenBank Accession LN501638 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K018601-022-276-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAACAAGAAGACACA
TAGAGACCCCTAAACAACCAGAATTAGAAAAATAATCGATTCCATCAATCAGAAGCCACT
TGACTCCCAGAGTTATCGCAGACCAGTTCGATCTTCGGCTTCTTTCTCTTTGTGCAATAC
CCCGTCGACGACAACCTAAGAAAGATCAAACCAACAAGTTTGTAGAATGCTCAGTAACAA
TAAAAGCTTGTAACAGAAGTTAGGGCTTTGTTTTGCAGTTTACCCCAACTGCAAGGAAGT
GTCGGAATGGTGCTCCTCGTTGTCATTCTCATCCTCTTCTGATGAAGAGCTCTCGGGATC
GGCTTCTGGGCTAGAATCTTGAGGCCTTTCATTCAACACTTTTGGTCCTGAACCTCTCCC
CAACATCTCAACCTGAGGGCAAATTAGAATTGAACTCAGTTATGACTGGTGAAGACCAGA
CCGAGCTAATATAAGGGTTTTGATGAATCTTTACCTGTTTGCGCAAGATTTGGTTTTCTT
CCAATGCTTTTTTCTCCTATTAATGACAAGGAA

Last Updated on 06.12.2018 16:11