Sequences from confirmation amplicon


Confirmation sequences for line 278B08 and hit At5g65510 are shown.
Line ID 278B08
Locus At5g65510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26187839
Primers used for PCR MZ21 - 8474, show primer details
   
Sequence Name 43-K018320-022-278-B08C-MZ21
GenBank Accession LN504676 [GenBank]
Sequencing primer gene specific primer, MZ21: 5'-TGTGTTGATTGATGCAGACATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K018320-022-278-B08C-MZ21
GCATCTATGGGATAACAGCTGTAGGAGGGAAGGTCAAGCCAGAAAAGGACGTCAAGGTAA
TAATCTCTCATTCTTTTGTTTCCTACGAGAAACAGTTTCTTGAATTATTTGCTTGGTTAC
TATGTTACTTTTGAATGTTTCGCATTGCAAAAACCTAATTTAGATAAACATGATCTCACT
TTCATGTGTTAATCTAACTTTTGTGTATATATATGCTAGCTAAAACCATTTTTATTGTCT
AGAGTCTTTGATAGAAACTAGTTTGATTTGGGTTAAAAACAAACATTTATTCTTTAACTT
TTATGGTTGGGGAAAACAAAAGCGGAAGCAGTACAAAGGTTTTGTCAAAGAAAGAGTGCC
AAGAGAAATGCATGCAATGGATTCAATAAATATAAATTACTGAAAAGTTTTTCCTTTTAG
GTGATATGTCTACTCTTTATGATGACTGATAGACTTTGGTATATGGGCATAATGTCAAGG
AAAGAACCAAATTTTAAACAGAAAGGACTAACAATAATAGAATAGAATTATGTAAATACA
TTTGGTTGAGTTTTTCATCACAAGGGTGCCGCCATTTTTGTTCCTTGTCTTTATTAGAGC
TATCTTTGAAAATCTTTCATTTCACCTTCTATGGGGACCCCAATGCTGATGCCTGTATGA
TGCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATA
   
Sequence Name 47-K018320-022-278-B08C-8409
GenBank Accession LN506042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K018320-022-278-B08C-8409
AGATTTCCTCGGACATGAAGCCATTTACATTGCATCATACAGGCATCAGCATTGGGGTCC
CCATAGAAGGTGAAATGAAAGATTTTCAAAGATAGCTCTAATAAAGACAAGGAACAAAAA
TGGCGGCACCCTTGTGATGAAAAACTCAACCAAATGTATTTACATAATTCTATTCTATTA
TTGTTAGTCCTTTCTGTTTAAAATTTGGTTCTTTCCTTGACATTATGCCCATATACCAAA
GTCTATCAGTCATCATAAAGAGTAGACATATCACCTAAAAGGAAAAACTTTTCAGTAATT
TATATTTATTGAATCCATTGCATGCATTTCTCTTGGCACTCTTTCTTTGACAAAACCTTT
GTACTGCTTCCGCTTTTGTTTTCCCCAACCATAAAAGTTAAAGAATAAATGTTTGTTTTT
AACCCAAATCAAACTAGTTTCTATCAAAGACTCTAGACAATAAAAATGGTTTTAGCTAGC
ATATATATACACAAAAGTTAGATTAACACATGAAAGTGAGATCATGTTTATCTAAATTAG
GTTTTTGCAATGCGAAACATTCAAAAGTAACATAGTAACCAAGCAAATAATTCAAGAAAC
TGTTTCTCGTAGGAAACAAAAGAATGAGAGATTATTACCTTGACGTCCTTTTCTGGCTTG
ACCTTCCCTCCTACAGCTGTTATCCCATAGATGCGCTTCGTATCTTCCAGTCCATCTATG
TCT

Last Updated on 06.12.2018 16:11