Sequences from confirmation amplicon


Confirmation sequences for line 279D02 and hit At1g69570 are shown.
Line ID 279D02
Locus At1g69570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26162827
Primers used for PCR MZ20 - 8474, show primer details
   
Sequence Name 58-K018604-022-279-D02C-MZ20
GenBank Accession LN509574 [GenBank]
Sequencing primer gene specific primer, MZ20: 5'-CTGACTGATGCTTACCAGTCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K018604-022-279-D02C-MZ20
CGAAGACTCCGAAGAACCGAAACTAAGGATCGTCCCCGAGTTATTATTATCACAATCCTC
AGAAGTGACTTGCAAGTAATGGTTTGAAGAAGGCCATCCTTTGTTCTTGCGACGACCTGA
GCCAACAGGAACGTTCCTCATAGATCCACCAGCTGTCCAATACCTCTGACAGTTCCTGCA
GAAGTAACGTGGCTGGTTCACGTTGTAGTTGTTGTAATAACAGAATTTGGTGTTTGCGCT
TTCACATCTAGGACATGGAATAAGCTTGTCCGGTTTCTTGAGAGCCGTTGATTTCTCTCC
TGATGTAGTACTTGTGGTAGTTGTTGTTGTGATCTCGCTCTGTTGATCACAGTTGTTCTT
GGAACTTCTTGGTGATGAAATCTCAGATACTGCTTTTGGTGGTTCGTTTAGATCGGATAA
TATATATGGATCTTTGAATTTGTTACTCTCTTGCTCACTTTTTTTAACCGGAACCTGAAT
TTTTTGTAGAATTTTGATTAGAATTAGCAAATGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGCTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTTTTATAAAATGAAAGTACATTTT
GATAAAACGACNAATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTT
   
Sequence Name 59-K018604-022-279-D02C-8409
GenBank Accession LN509858 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K018604-022-279-D02C-8409
TAGATTTCCCGGACATGAAGCCATTTACATTTGCTAATTCTAATCAAAATTCTACAAAAA
ATTCAGGTTCCGGTTAAAAAAAGTGAGCAAGAGAGTAACAAATTCAAAGATCCATATATA
TTATCCGATCTAAACGAACCACCAAAAGCAGTATCTGAGATTTCATCACCAAGAAGTTCC
AAGAACAACTGTGATCAACAGAGCGAGATCACAACAACAACTACCACAAGTACTACATCA
GGAGAGAAATCAACGGCTCTCAAGAAACCGGACAAGCTTATTCCATGTCCTAGATGTGAA
AGCGCAAACACCAAATTCTGTTATTACAACAACTACAACGTGAACCAGCCACGTTACTTC
TGCAGGAACTGTCAGAGGTATTGGACAGCTGGTGGATCTATGAGGAACGTTCCTGTTGGC
TCAGGTCGTCGCAAGAACAAAGGATGGCCTTCTTCAAACCATTACTTGCAAGTCACTTCT
GAGGATTGTGATAATAATAACTCGGGGACGATCCTTAGTTTCGGTTCTTCGGAGTCTTCG
GTTACAGAGACTGGTA

Last Updated on 06.12.2018 16:11