Sequences from confirmation amplicon


Confirmation sequences for line 280E07 and hit At5g19010 are shown.
Line ID 280E07
Locus At5g19010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6346711
Primers used for PCR 33by - 8474, show primer details
   
Sequence Name 24-K108417-0022-280-E07C-33by
GenBank Accession LN497024 [GenBank]
Sequencing primer gene specific primer, 33by: 5'-GCCTGAGGTAACACATAAAACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108417-0022-280-E07C-33by
GGAGTGTAAAGAACGAAATGTATGAACCACCTCTTCAGCTGTAGGCCGGCCCTTGGGTCT
CCAAGACAACATCTTTTCTAGAAGACGAAGAGCCAGAGGATCAGTATGTGGGAACTTATG
TGAAAAAGGAATAGGCTTCTTCTTCCTCATGCTGCTCAAGTATCTTCGAGCCTTCTCGTT
CCTAACCTGTTTCAGTGTAAGAAATTCAGCCAAAAAAACAAACAATGCTTAAGCATTGTG
GCAAGGAACACATACCCTTCCAATGGCTTCAGCCGATGGAGTTCCC
   
Sequence Name 08-K108416-0022-280-E07C-8409
GenBank Accession LN489766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108416-0022-280-E07C-8409
CATTTACATTTAATACTATCAGTGTTAGGTCGGTGATCTGGATTTAGGCTCTGTAGATTT
TGTATATATACTTTCATCGAATGTTTCAAATTTTCATAGGGAATCTAGTCAGTAGACACT
ATAGCTCTCTCATTCAGATTATAAAATTTTATGGTAACATTTTTTTTCTTTATCTATGCT
TCTGTTCAGTATACACCGGCAATAGATATATGGAGCATCGGGTGCATTTTTGCAGAACTC
TTAACAGGGAAACCTCTTTTCCCCGGGAAAAATGTGGTCCATCAATTGGATCTGATGACG
GATATGCTGGGAACTCCATCTGCTGAAGCCATTGGAAGGGTATGTGTTCCTTTCCACAAT
GCTTAAGCATTGTTTCTTTTTTTGGCTGAATTTCTTACACTGAAACGGGTTAGGAACGAG
AAAGCTCGAAGATACTTGAGCAGCATGAGGAAGAAGAAGCCTATTCCTTTTTCACATAAG
TTCCCACATACTGATCCTCTTGCTCTTCGTCTTCTAGAAAAGATGTTGTCTTTTGAACCC
ACGGACCGGCCTACCGCCGAAGAGGTGGTCTCTACCTTTCTTTCTTTACCCCCCTTATGA

Last Updated on 06.12.2018 16:11