Sequences from confirmation amplicon


Confirmation sequences for line 280F04 and hit At2g43550 are shown.
Line ID 280F04
Locus At2g43550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18073341
Primers used for PCR KS12 - 8474, show primer details
   
Sequence Name 43-K023624-022-280-F04C-8409
GenBank Accession LN504702 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K023624-022-280-F04C-8409
ATTCCCGGACATGAAGCCATTTACAATTGGTAATTACTCTTTCTTCCTCGTCGTTCTTGG
TAAGTACTGAATCTATATACAGTTACTGCATTATTTACGATATTACCCACTCTTACAACC
TCATAACAATATGCAGAGGCCCCGGAGAAGATAGAAGCCAAAGACGACAAGTTCATATGT
GTGGTTGAGTATGGAGGAGACGTGGGTCCAACGTTCTGTAACCCTAAATTCTTCCCCACG
TTGTGCCGTCAAAACTGCCGGAGCTTCAAAGGTGCCAAAGGCGGTAAATGCGTGAAGCAA
CCCAAACACAAACATATCAAGTGCTTCTGCGACTACTGTAAAGACGA
   
Sequence Name 44-K023624-022-280-F04C-KS12
GenBank Accession LN505049 [GenBank]
Sequencing primer gene specific primer, KS12: 5'-GTCGTCTTTACAGTAGTCGCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K023624-022-280-F04C-KS12
CTTGATATGTTTGTGTTTGGGTTGCTTCACGCATTTACCGCCTTTGGCACCTTTGAAGCT
CCGGCAGTTTTGACGGCACAACGTGGGGAAGAATTTAGGGTTACAGAACGTTGGACCCAC
GTCTCCTCCATACTCAACCACACATATGAACTTGTCGTCTTTGGCTTCTATCTTCTCCGG
GGCCTCTGCATATTGTTATGAGGTTGTAAGAGTGGGTAATATCGTAAATAATGCAGTAAC
TGTATATAGATTCAGTACTTACCAAGAACGACGAGGAAGAAAGAGTAATTACCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAAGAGTAATTTACCAATTTTTT

Last Updated on 06.12.2018 16:11