Sequences from confirmation amplicon


Confirmation sequences for line 280G04 and hit At1g19940 are shown.
Line ID 280G04
Locus At1g19940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6918886
Primers used for PCR ST01 - 8474, show primer details
   
Sequence Name 07-K026236-022-280-G04C-8409
GenBank Accession LN489109 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026236-022-280-G04C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGTAAGCTATGGAATGCCCTGCAACACCCT
GTGTCCTCTGCATTTCTAGCTACACTCTACAGTGACTATATGCTCACCTCTGGTGTTAAG
GAACTATCTTGCAGTGACCAATCCTTTAAACCTTCAGATCTCAGAAAATTCGCTAGATCT
CAGGTCCATATGCATATTAATTTGGTTTCTTATTTTTCCTCTTAATTAAACGTATAATAC
TGTAACTTGATATATGCATATATAATGTGCAGGCTGATTACATGTTAGGGAAAAATCCGG
AGAAAATGAGCTATTTGGTGGGATATGGTGAGAAATATCCAGAATTTGTGCATCATAGAG
GAGCTCCAATAC
   
Sequence Name 08-K026236-022-280-G04C-ST01
GenBank Accession LN489573 [GenBank]
Sequencing primer gene specific primer, ST01: 5'-GGTATTGAAGCTCCTCTATGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K026236-022-280-G04C-ST01
CAAATTCTGGATATTTCTCACCATATCCCACCAAATAGCTCATTTTCTCCGGATTTTTCC
CTAACATGTAATCAGCCTGCACATTATATATGCATATATCAAGTTACAGTATTATACGTT
TAATTAAGAGGAAAAATAAGAAACCAAATTAATATGCATATGGACCTGAGATCTAGCGAA
TTTTCTGAGATCTGAAGGTTTAAAGGATTGGTCACTGCAAGATAGTTCCTTAACACCAGA
GGTGAGCATATAGTCACTGTAGAGTGTAGCTAGAAATGCAGAGGACACAGGGTGTTGCAG
GGCATTCCATAGCTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11