Sequences from confirmation amplicon


Confirmation sequences for line 282H08 and hit At5g09330 are shown.
Line ID 282H08
Locus At5g09330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2893543
Primers used for PCR TD01 - 8474, show primer details
   
Sequence Name 19-K027344-022-282-H08C-8409
GenBank Accession LN494614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K027344-022-282-H08C-8409
ATTTCATTGAATAATCCTGAATGCAAAAAAAAACTATCCATCAAATAGATCCAACATTGA
ATATAAGTCATCATTGTTGGGGGTCTGAACGGTCTCTGCCAGAGGTGCAGTAGACATAGG
AGTGGTAGCTGCATCACTTGGATGTGGAAGCTCGCTGGTGGTATTAGTTGTAGCTGGTAG
GAAATCCGAGACACATGACTCTGATATCATGCCACCAAAACAATCCTTATTGGAGTCATC
TGGGGCAGTCAAAGCCAGAGTGGTTTCTTTGCTAGGCTCAAGAAGGTTTGTGGGATTACT
AGTAGAAGGAACATCAGTACGTACTTCCTCATCACTCCAGTCCTCTTCCTTAAACGGAGC
TCCATATTGAGCTCCATTTCTAGGTCCAGGACCATCTTTCTTGAACAGAACACACACCAC
ATAAGTATCCTGCAGTCCAAAACATAATTGTAAGTAAAGAGAGAGTGATATAAGCTTACA
TGGATGATACATAAATCTATAAAACTAAACAAGCAGGAAACAATATATAAGTGGAAGAAG
GAAAAAATTGCAGGCAG
   
Sequence Name 20-K027344-022-282-H08C-TD01
GenBank Accession LN495065 [GenBank]
Sequencing primer gene specific primer, TD01: 5'-TTGATAGTGTGTTACTGTCCTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K027344-022-282-H08C-td1
CTTATATATTGTTTCCTGCTTGTTTAGTTTTATAGATTTATGTATCATCCATGTAAGCTT
ATATCACTCTCTCTTTACTTACAATTATGTTTTGGACTGCAGGATACTTATGTGGTGTGT
GTTCTGTTCAAGAAAGATGGTCCTGGACCTAGAAATGGAGCTCAATATGGAGCTCCGTTT
AAGGAAGAGGACTGGAGTGATGAGGAAGTACGTACTGATGTTCCTTCTACTAGTAATCCC
ACAAACCTTCTTGAGCCTAGCAAAGAAACCACTCTGGCTTTGACTGCCCCAGATGACTCC
AATAAGGATTGTTTTGGTGGCATGATATCAGAGTCATGTGTCTCGGATTTCCTACCAGCT
ACAACTAATACCACCAGCGAGCTTCCACATCCAAGTGATGCAGCTACCACTCCTATGTCT
ACTGCACCTCTGGCAGAGACCGTTCAGACCCCCAACAATGATGACTTATATTCAATGTTG
GATCTATTTGATGGATAGTTTTTTTTTGCATTCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11