Sequences from confirmation amplicon


Confirmation sequences for line 284B09 and hit At1g66930 are shown.
Line ID 284B09
Locus At1g66930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24970730
Primers used for PCR 26qa - 8474, show primer details
   
Sequence Name 41-K115305-0022-284-B09C-26qa
GenBank Accession LN504193 [GenBank]
Sequencing primer gene specific primer, 26qa: 5'-GGGACAAAGATCGTTGAGATAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115305-0022-284-B09C-26qa
TATTAAGAATTCTGAATCACGGAGGCTATGTTAAGCTCTGCGAATCCTCCGCTACAGTCA
AGCTTGAAGTCAGGGTGGCCACAATATTCTCGACCAGGTATCCAAAAGGGGTAATAGAGA
TTTCTCTGATTGCCGCAGCTAAACCGTTCACTACAGCGGCGCCTAAGCTCATCAGCTGAA
ACATAAAACAAAAATGGGATCACAAAGAGCATCATTTGACTTTAAGACAGAGAATTCGTC
AACAACAGAGATAGCTTGATCATTTTGTTATACTCTTGTTTTGTTTCCTTGGAGGCCACT
CAATAGGAACTATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 06-K115304-0022-284-B09C-8409
GenBank Accession LN488941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115304-0022-284-B09C-8409
GTATAGTTCCTATTGAGTGGCCTCCAAGGAAACAAAACAAGAGTATAACAAAATGATCAA
GCTATCTCTGTTGTTGACGAATTCTCTGTCTTAAAGTCAAATGATGCTCTTTGTGATCCC
ATTTTTGTTTTATGTTTCAGCTGATGAGCTTAGGCGCCGCTGTAGTGAACGGTTTAGCTG
CGGCAATCAGAGAAATCTCTATTACCCCTTTTGGATACCTGGTCGAGAATATTGTGGCCA
CCCTGACTTCAAGCTTGACTGTAGCGGAGGATTCGCAGAGCTTAACATAGCCTCCGTGAA
TTTCAGAATCTTAAATATGAGTTATGACTCTTCTAACAAAAGACTTGCCAGATCAGACTA
TCTCAACGACNTTTGTCC

Last Updated on 06.12.2018 16:11