Sequences from confirmation amplicon


Confirmation sequences for line 284F04 and hit At3g06710 are shown.
Line ID 284F04
Locus At3g06710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2119700
Primers used for PCR 03vq - 8474, show primer details
   
Sequence Name 26-K117953-0022-284-F04C-03vq
GenBank Accession LN498118 [GenBank]
Sequencing primer gene specific primer, 03vq: 5'-CCATCAGCATATGTGTGTCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117953-0022-284-F04C-03vq
CATAGCAATTGTAGGAGGTGGCGGTTTATTCGAAAACTCACCTGTCATTCTTGGATACAT
GATGACGCTGTCAATTCCCTTTGCCTACTTAGGAATTTTCCTCACTCTGCGTCAAATCTC
GTTTCCAGCCATAGTCAGGTGGTTTTCCTTGGGATATCACTTCATAACAAAGAAACTGTC
CTGCCTCCTGAGCAAAAATCTCGTTTCCTGTCAACTGTTTCGCGACTAAGGCATGGCGGG
GAAAGTTCAAGAGAATCAGAAACGTGGGAATCAATAAGCTTCCTTCAATGTGGCTGCTTC
AAGATGTGATTGGTTCAATAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGACAAGGTTATT
   
Sequence Name 02-K117952-0022-284-F04C-8409
GenBank Accession LN487222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117952-0022-284-F04C-8409
TCGGACATGAGCCATTTCATTGAATATATCCTGCTTATTGAACCAATCACATCTTGAAGC
AGCCACATTGAAGGAAGCTTATTGATTCCCACGTTTCTGATTCTCTTGAACTTTCCCCGC
CATGCCTTAGTCGCGAAACAGTTGACAGGAAACGAGATTTTTGCTCAGGAGGCAGGACAG
TTTCTTTGTTATGAAGTGATATCCCAAGGAAAACCACCTGACTATGGCTGGAAACGAGAT
TTGACGCAGAGTGAGGAAAATTCCTAAGTAGGCAAAGGGAATTGACAGCGTCATCATGTA
TCCAAGAATGACAGGTGAGTTTTCGAATAAACCGCCACCTCCTACAATTGCTATTGCAAT
CCAGCGAAAAGAAAACGGCAGAAGAATAAAGAAGAAAGACACACATATGNCTGATGG
Primers used for PCR 38vq - 8474, show primer details
   
Sequence Name 27-K117953-0022-284-F04C-38vq
GenBank Accession LN498583 [GenBank]
Sequencing primer gene specific primer, 38vq: 5'-GGTTTGTGCTTGTTTTATGGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117953-0022-284-F04C-38vq
TCTCATTGCAAGCTTCTTCGGGGAACCTCCTAGGCTAGTTGGTGTTGCTGTTCACGAACT
TGGAGCCATTGGACGGCTGCTTTTCTTTCTGGATAACAAATCTTTTGATGTAAGTCTAAT
GTCCCTTCTAGTTCTCTTCCTTCGTCCACCCTATGTAACTTAATTACCATATGGAGCATG
TTTTGAATAACAGGTTCTGTTTGTTATCTTATCTACAGGCTCTTACCATCAGCATATGTG
TGTCTTTCTTCTTTATTCTTCTGCCGTTTTCTTTTCGCTGGATTGCAATAGCAATTGTAG
GAGGTGGCGGTTTATTCGAAAACTCACCTGTCATTCTTGGATACATGATGACGCTGTCAA
TTCCCTTTGCCTACTTAGGAATTTTCCTCACTCTGCGTCAAATCTCGTTTCCAGCCATAG
TCAGGTGGTTTTCCTTGGGATATCACTTCATAACAAAGAAACTGTCCTGCCTCCTGAGCA
AAAATCTCGTTTCCTGTCAACTGTTTCGCGACTAAGGCATGGCGGGGAAAGTTCAAGAGA
ATCAGAAACGTGGGAATCAATAAGCTTCCTTCAATGTGGCTGCTTCAAGATGTGATTGGT
TCAATAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACCGGTTATT
   
Sequence Name 03-K117952-0022-284-F04C-oriL
GenBank Accession LN487671 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117952-0022-284-F04C-oriL
TCATTGAATATATCCTGCTTATTGAACCATCACATCTTGAAGCAGCCACATTGAAGGAAG
CTTATTGATTCCCACGTTTCTGATTCTCTTGAACTTTCCCCGCCATGCCTTAGTCGCGAA
ACAGTTGACAGGAAACGAGATTTTTGCTCAGGAGGCAGGACAGTTTCTTTGTTATGAAGT
GATATCCCAAGGAAAACCACCTGACTATGGCTGGAAACGAGATTTGACGCAGAGTGAGGA
AAATTCCTAAGTAGGCAAAGGGAATTGACAGCGTCATCATGTATCCAAGAATGACAGGTG
AGTTTTCGAATAAACCGCCACCTCCTACAATTGCTATTGCAATCCAGCGAAAAGAAAACG
GCAGAAGAATAAAGAAGAAAGACACACATATGCTGATGGTAAGAGCCTGTAGATAAGATA
ACAAACAGAACCTGTTATTCAAAACATGCTCCATATGGTAATTAAGTTACATAGGGTGGA
CGAAGGAAGAGAACTAGAAGGGACATTAGACTTACATCAAAAGATTTGTTATCCAGAAAG
AAAAGCAGCCGTCCAATGGCTCCAAGTTCGTGAACAGCAACACCAACTAGCCTAGGAGGT
TCCCCGAAGAAGCTTTGCAATAGAGAATAATACCACTCACAAACGATCTTGTTATATTTC
CATAAAACAAGCACAAACC

Last Updated on 06.12.2018 16:11