Sequences from confirmation amplicon


Confirmation sequences for line 285G03 and hit At1g30070 are shown.
Line ID 285G03
Locus At1g30070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10548159
Primers used for PCR PF43 - 8474, show primer details
   
Sequence Name 66-K103791-0022-285-G03C-PF43
GenBank Accession LN511594 [GenBank]
Sequencing primer gene specific primer, PF43: 5'-GTTTTTATCTCCCTCTTTATCGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K103791-0022-285-G03C-PF43
GTCTCTCCTTTTTTCGGGGCTCGAGGAATGAGTTGAAATTGTTGATTATCACTATCCACA
TTTATCCAAAGTTATACTTAAGCATATAAAAATGAGAAAATTGCTTTATATTTTGGTAAA
GTAGGACGTAACTCGAAGATCCTTTTAAATGGAAAAAAATAATAGAATCTAAAACTTTTT
TTTTATTGCTTCGTAAAATAAAATAGTAGAATCTAGAATAAAATTTATTTTCCTATAGAA
TATCAATTTAAAGATGTGACTGATAATTTTTCCCTTTTTTTTAGAGTACATAATTAAGTG
GACAGAAAAATATGTAGGACCAAAGTTTTAGATTATTTTCCAGTAACTTTAATATAGAGT
GAAAGTTTCTGCTGAAAATAAAAAAATTGTTTGATAACCAAATGTTTTACTAAATGGGCT
TGGCTCTGTATGAAAGAAGAAAGCCCAAGAAGCTAAAGCATCAAAAAGCTCAATATGAGG
TCTTCTAGTACTTTCTAGTTTCCACAAGACTCTCTCGTTCCCTTCGCTTTGTGCTTGATC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCNNAAA
TGTAGATGTC
   
Sequence Name 82-K103792-0022-285-G03C-8409
GenBank Accession LN514184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K103792-0022-285-G03C-8409
TTTACATTGAATATATCCTGATCAAGCACAAAGCGAAGGGAACGAGAGAGTCTTGTGGAA
ACTAGAAAGTACTAGAAGACCTCATATTGAGCTTTTTGATGCTTTAGCTTCTTGGGCTTT
CTTCTTTCATACAGAGCCAAGCCCATTTAGTAAAACATTTGGTTATCAAACAATTTTTTT
ATTTTCAGCAGAAACTTTCACTCTATATTAAAGTTACTGGAAAATAATCTAAAACTTTGG
TCCTACATATTTTTCTGTCCACTTAATTATGTACTCTAAAAAAAAGGGAAAAATTATCAG
TCACATCTTTAAATTGATATTCTATAGGAAAATAAATTTTATTCTAGATTCTACTATTTT
ATTTTACGAAGCAATAAAAAAAAAGTTTTAGATTCTATTATTTTTTTCCATTTAAAAGGA
TCTTCGAGTTACGTCCTACTTTACCAAAATATAAAGCAATTTTCTCATTTTTATATGCTT
AAGTATAACTTTGGATAAATGTGGATAGTGATAATCAACAATTTCAACTCATTCCTCGAG
CCCCGAAAAAAAGGAGAGACAATTGTCGCCATTTTTGGCTTGATGCCCGATAAAGAGGGA
GATAAAA

Last Updated on 06.12.2018 16:11