Sequences from confirmation amplicon


Confirmation sequences for line 285G08 and hit At5g01840 are shown.
Line ID 285G08
Locus At5g01840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:324334
Primers used for PCR MM58 - 8474, show primer details
   
Sequence Name 04-K020358-022-285-G08C-8409
GenBank Accession LN487733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K020358-022-285-G08C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAATGGCCAACATAATCTAACA
CAAAATCAATGAAAATGGAGAGAGCATCTCACTCACCTTATAATAACCTTTATATTTTCC
ACGTATGTGGGCCCCCTCACATAAACCTCTCTTTCCTTTATAAATTCTCCACTCTTCACA
CTCTCACTTTTGTTCATGTACCAAAGAAGTCCCCAACAAACACAAAATAATCAACAGAGA
GAGTCAGAGAGATAATGGGTAATAACTATCGGTTTAAGCTATCGGAGTTAATCCCTAACG
CTTGGTTTTACAAACTTAGAGACATGTCCAAATCCAAGAAGAAGAATCTTCAGAGCCAAC
CAAATAGCACCACCTCCAAGAAGAAACACCACGCAGTCCCTACTCCTACGTCCACAACGC
CACTCAGTCCTAGGCCTCCTAGGCGCCCTTCTCACTCTTCCAAAGCGCCACCCTCCCATC
CTCCCAGGAAGTCCTCCGGGAACCGTCTTCGTCACCGAGCCACCGTCGACTCTAAGTCTT
CCACGACTTCCGGCGACAGCACTACCACAGAAACAGGTTCTTTCTCACCGGACTTTCGCT
CGGATCAAGTTCTCCTCCCCGACGAGTCATTAACGGGCTCCTGGCACAGCCCTTGCAGCT
CCAAGTTATCCAAAACCGCAACCTTTACGCCGCCGCCGGAACTCGAACTCCGGCCCATCA
TTACCAANAACGGCCGCCACCGCAC
   
Sequence Name 08-K020358-022-285-G08C-MM58
GenBank Accession LN489530 [GenBank]
Sequencing primer gene specific primer, MM58: 5'-TCGACTCCTTAAAGTCTCTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K020358-022-285-G08C-MM58
CCTTCACCACCGCCCTGCTCCGCCGAGCCGACGACCCGCTTCTTCTTGCGCTGCTGCTCA
CCGAGATCCGCGGCGACCGCATTCTAAGTCTCACCCCCGCCGGAGAATTCACCGCCGTTT
TCCGTGCGGTGGCGGCCGTTTTGGTAATGATGGGCCGGAGTTCGAGTTCCGGCGGCGGCG
TAAAGGTTGCGGTTTTGGATAACTTGGAGCTGCAAGGGCTGTGCCAGGAGCCCGTTAATG
ACTCGTCGGGGAGGAGAACTTGATCCGAGCGAAAGTCCGGTGAGAAAGAACCTGTTTCTG
TGGTAGTGCTGTCGCCGGAAGTCGTGGAAGACTTAGAGTCGACGGTGGCTCGGTGACGAA
GACGGTTCCCGGAGGACTTCCTGGGAGGATGGGAGGGTGGCGCTTTGGAAGAGTGAGAAG
GGCGCCTAGGAGGCCTAGGACTGAGTGGCGTTGTGGACGTAGGAGTAGGGACTGCGTGGT
GTTTCTTCTTGGAGGTGGTGCTATTTGGTTGGCTCTGAAGATTCTTCTTCTTGGATTTGG
ACATGTCTCTAAGTTTGTAAAACCAAGCGTTAGGGATTAACTCCGATAGCTTAAACCGAT
AGTTATTACCCATTATCTCTCTGACTCTCTCTGTTGATTATTTTGTGTTTGTTGGGGACT
TCTTTGGTACATGAACAAAAGTGAGAGTGTGAAGAGTGGAGAATTTA

Last Updated on 06.12.2018 16:11